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PCR is the most widely used technique in molecular biology. New PCR variants offering substantial benefits to existing protocols appear on a frequent basis. PCR: Methods Express describes the very latest PCR-based methodologies and approaches to provide the most up-to-date practical advice on how to tackle a broad range of biological problems including: *real time qRT-PCR *rapid generation of gene targeting constructs *PCR multiplexes *PCR-based mutagenesis *identification of microdeletions and microduplications *DNA methylation analysis *forensic genetic DNA typing *genotyping *identification of mutations in single cells *whole genome amplification *diagnosis of infectious diseases *inverse PCR-based RFLP This book is a comprehensive research guide; every chapter discusses the merits and limitations of the available approaches and then provides fully-proven protocols with hints and tips for success. PCR: Methods Express is an essential laboratory manual for researchers in all life science fields and at all levels, from postgraduate student to principal investigator.
Chuck Hines enjoyed a 40-year career with the YMCA, during which he was a strong advocate of the Olympic sport of water polo. He was a three-time All-America player, and he coached teams at three YMCAs that won national championships. His teams all started out at the beginning level, in small pools and with insufficient equipment, and fought their way to the top. This book is the story of those teams and their rags to riches achievements.
Contains up-to-date information on the full range of international schools, including single-sex, co-educational, day and boarding schools, this guide will assist parents and children in choosing the right international school for them.
This guide has been designed with the reader inmind. In the editorial section are articles written by experts in their field covering a wide variety of issues parents are likely to come across when choosing a school for their child. The dirctories contain basic information about all the schools in each country complete with contact details. Some schools provide more information on what they offer and include photographs. In the appendix is up-to-date information about international curricula and the examinations, tests and qualifications available, cobntact details for Ministries of Education worldwide and a useful list of educational acronyms and abbreviations.
Chuck Hines, an All-America athlete in his younger days, enjoyed a 40-year career with the YMCA, during which he taught 15,000 children to swim and coached numerous national champions, some of whom became gold, silver, and bronze medalists in Olympic and World competition. He received recognition from the YMCA as a Distinguished Director of Physical Education; was inducted into the Western North Carolina Sports Hall of Fame; earned the Western North Carolina Humanitarian award; and carried the Olympic Torch. In this book, he recounts his YMCA adventures and explains why it is such a cherished and popular international organization.
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WINNER OF THE BOOKER PRIZE FOR FICTION 2013 It is 1866, and Walter Moody has come to make his fortune upon the New Zealand goldfields. On arrival, he stumbles across a tense gathering of twelve local men, who have met in secret to discuss a series of unsolved crimes. A wealthy man has vanished, a whore has tried to end her life, and an enormous fortune has been discovered in the home of a luckless drunk. Moody is soon drawn into the mystery: a network of fates and fortunes that is as complex and exquisitely patterned as the night sky. The Luminaries is an extraordinary piece of fiction. It is full of narrative, linguistic and psychological pleasures, and has a fiendishly clever and original structuring device. Written in pitch-perfect historical register, richly evoking a mid-19th century world of shipping and banking and goldrush boom and bust, it is also a ghost story, and a gripping mystery. It is a thrilling achievement and will confirm for critics and readers that Catton is one of the brightest stars in the international writing firmament.