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DNA polymerases in Biotechnology
DNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have accelerated DNA polymerase engineering for biotechnology. For example, DNA polymerases have been engineered for increased speed and fidelity in PCR while lowering amplification sequence bias. Inhibitor resistant DNA polymerase variants enable PCR directly from tissue (i.e. blood). Design of DNA polymerases that efficiently incorporate modified nucleotide have been critical for development of next generation DNA sequencing, synthetic biology and other labeling and detection technologies. The Frontiers in Microbiology Research Topic on DNA polymerases in Biotechnology aims to capture current research on DNA polymerases and their use in emerging technologies.
DNA Replication Origins in Microbial Genomes
DNA replication, a central event for cell proliferation, is the basis of biological inheritance. Complete and accurate DNA replication is integral to the maintenance of the genetic integrity of organisms. In all three domains of life, DNA replication begins at replication origins. In bacteria, replication typically initiates from a single replication origin (oriC), which contains several DnaA boxes and the AT-rich DNA unwinding element (DUE). In eukaryotic genomes, replication initiates from significantly more replication origins, activated simultaneously at a specific time. For eukaryotic organisms, replication origins are best characterized in the unicellular eukaryote budding yeast Saccha...
Bioinformatics and Computational Biology
Bioinformatics and Computational Biology: Technological Advancements, Applications and Opportunities is an invaluable resource for general and applied researchers who analyze biological data that is generated, at an unprecedented rate, at the global level. After careful evaluation of the requirements for current trends in bioinformatics and computational biology, it is anticipated that the book will provide an insightful resource to the academic and scientific community. Through a myriad of computational resources, algorithms, and methods, it equips readers with the confidence to both analyze biological data and estimate predictions. The book offers comprehensive coverage of the most essenti...
Annual Convocation
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The Blues of Portsmouth P.D.
The Case of the Erroneous Homophone In the first of two episodes of The Blues of Portsmouth PD, your author introduces the nine fictitious but charismatic members of the Portsmouth, New Hampshire, Police Department: Chief Wesley Frey, fair and definitely in charge but facing retirement; the veterans, Desk Sgt. Joe Cooper and dispatcher and sergeant Sally Thorne, who have a love-hate relationship, add comic relief; Detective Lt. Tim Slone, who has a stammer until he deals with pressure; Detective Beth “Skiv” Van Dyke and her lover, genealogist turned detective Keegen “Keeg” O’Regan; Detective Tony Brock, who arrives from another jurisdiction; coroner Dr. Newt Brookings, an African American who loves his work; and medical examiner Dr. Alex Long, a small man with many talents. All is calm in Portsmouth until three bodies indicate that they are facing a serial killer.
