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Dendritic Cell Protocols
Dendritic Cell Protocols provides chapter and verse for many useful practical approaches to the art of studying dendritic cells. The book gives information on the usual techniques for derivation of human dendritic cells from precursor stem cells, such as monocytes. In addition it provides data on the difficult tasks of isolating dendritic cells directly from different tissues; whether dendritic cells from precursor cells or from tissues of mouse or human are required, this book contributes practical information. The last section of the book is devoted to functional aspects of dendritic cells ranging from inf- mation relevant to cell migration to antigen uptake and T cell stimulation. But wha...
Dendritic Cell Protocols. Methods in Molecular Medicine
Stephen P Robinson, MD, PhD, and Andrew Stagg, PhD, have brought together a wide range of time-proven methods for studying these dendritic cells. Many of these readily reproducible techniques deal with the problem of obtaining sufficient dendritic cells for analysis, whether by isolation from a wide vareity of tissues, or from various progenitor cell populations. Other methods describe in step-by-step fashion the techniques commonly used for analyzing aspects of dendritic cells, ranging from cell migration to antigen uptake and T cell stimulation. In addition, a few techniques explore the practical challenges involved in using dendritic cells in a clinical setting to develop novel immunotherapeueutics.
Gut Ecology
This new book brings together for the first time a world-class series of experts to explore this intricate system. These scientists and clinicians explore the traditional bacteriological and novel molecular techniques, which are now being used to define previously unknown bacterial species. The nature of bacterial epithelial interactions in health and disease is investigated. Specific defence mechanisms such as defensins, the epithelial immune system and cytokine regulation are covered. The modification of this bacterial environment may have a valuable role in preventing and treating disease - the effects of prebiotics, food, and probiotic bacteria are defined. Lastly, the areas of proven clinical benefit from the use of probiotic bacteria, and their mechanisms of action, are discussed.
Human Airway Inflammation
Rubor (redness), tumor (swelling), calor (heat), and dolor (pain) are the classical signs of inflammation. These features are obvious in the skin, where injury or disease causes flare, wheal, and painful burning sensations. Vasodi- tation underlies the flare and heat, plasma exudation the swelling, and acti- tion of sensory nerves relays pain. In chronic conditions, skin biopsies show inflammatory cell infiltrate. Inflammation is not unique to the skin and contr- utes to disease and repair processes in other organ systems in the body. From the viewpoint of this volume, lung inflammation is now recognized as central to the pathophysiology of a number of severe respiratory conditions, the two ...
Drugs of Abuse
Drugs of Abuse: Neurological Reviews and Protocols is intended to provide insightful reviews of key current topics and, particularly, state-- the-art methods for examining drug actions in their various neuroanato- cal, neurochemical, neurophysiological, neuropharmacological, and molecular perspectives. The book should prove particularly useful to n- comers (graduate students and technicians) in this field, as well as to those established scientists (neuroscientists, biochemists, and molecular biologists) intending to pursue new careers or directions in the study of drugs. The book’s protocols cover a wide variety of coherent methods for gathering inf- mation on quantitative changes in prot...
Lung Cancer
The Methods in Molecular Medicine series is intended as a resource for both novice and experienced investigators attempting to diversify their tech- logical base in research. Lung Cancer: Volume 1: Molecular Pathology Me- ods and Reviews presents an overview of the current status of assays employed to detect and characterize the multitude of pathologies that contribute to the development of this deadly disease. As with all volumes in the Methods in Molecular Medicine series, the reader should find that each methods-based chapter provides clear instructions for the performance of various protocols, supplemented by additional technical notes that provide valuable insight. These notes are desig...
Dendritic Cells in Fundamental and Clinical Immunology
These Proceedings contain the contributions of the participants of the Second International Symposium on Dendritic Cells that was held from the 1st to 25th of June 1992 in Amsterdam, the Netherlands. The First International Symposium on Dendritic Cells was organized as a Satellite symposium at the occasion of the 30th anniversary of the Japanese Reticuloendothelial Society by Dr. Y. Imai in Yamagata (Japan), in 1990. It was entitled "Dendritic Cells in Lymphoid Tissues," and focused primarily on the Interdigitating Cells (IDC), Epidermal Langerhans cells (LC) and Follicular Dendritic Cells (FDC) , from the point of view of human pathology. However, the concept of Dendritic Cell System, compr...
Regulation of Bacterial Virulence
A comprehensive compendium of scholarly contributions relating to bacterial virulence gene regulation. • Provides insights into global control and the switch between distinct infectious states (e.g., acute vs. chronic). • Considers key issues about the mechanisms of gene regulation relating to: surface factors, exported toxins and export mechanisms. • Reflects on how the regulation of intracellular lifestyles and the response to stress can ultimately have an impact on the outcome of an infection. • Highlights and examines some emerging regulatory mechanisms of special significance. • Serves as an ideal compendium of valuable topics for students, researchers and faculty with interests in how the mechanisms of gene regulation ultimately affect the outcome of an array of bacterial infectious diseases.
Measuring Immunity
Most of the diseases of modern mankind involve either acute or chronic inflammation. Measuring Immunity integrates the current information available on biomarkers and surrogate assays into a single handbook. It highlights the principles behind various applications, gives a brief summary on how they are conducted and provides detailed and critical analyses of murine models of immunity, clinical trials, and tests to predict utility and benefit. Measuring Immunity is indispensable for scientists and clinicians interested in the clinical applications of modern immunobiology. * Defines which assays of immune function are helpful in the assessment of clinical disorders involving inflammation and immunity* Assesses the dynamics of cellular and soluble factors in the peripheral blood using modern techniques * Includes basic science foundations as well as the approaches currently applied
