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This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
Electrical power system, electronic system, telecommunication system, control system, informatics, Educational Issues in Electrical Engineering, and other topics related to recent development in electrical engineering
This edited volume is based on the best papers accepted for presentation during the 1st Springer Conference of the Arabian Journal of Geosciences (CAJG-1), Tunisia 2018. The book is of interest to all researchers in the fields of petroleum engineering, reservoir engineering and petroleum geochemistry. The MENA region accounts for more than 50 percent of the world's hydrocarbon reserves. Despite being the largest oil and gas producer of the world, the MENA countries face routine problems regarding petroleum engineering, reservoir modelling and production optimization. This volume offers an overview of the latest information and ideas regarding reservoir engineering, petrophysical engineering, petroleum system modelling, non-conventional energy resources and environmental impact of oil production. Main topics include: 1. Advances in petrophysical characterization of reservoir rocks2. Enhanced oil recovery methods 3. Advances in petroleum exploration and management 4. Evaluation of hydrocarbon source potential and petroleum system modeling5. Non-conventional energy resources
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When fifteen-year-old Anna begins receiving messages from another time, her parents take her to the doctor. But he can find nothing wrong; in fact he believes there may be some truth to what she is seeing. Anna is haunted by visions of the desolate world of 2082. She sees her great-granddaughter, Nova, roaming through wasteland with a band of survivors, after animals and plants have died out. The more Anna sees, the more she realises she must act to prevent the future in her visions becoming real. But can she act quickly enough? 'Compelling' Sunday Times
Just like the Gutenberg revolution in the fifteenth century, which led to the emergence of non-conventional religious authority in the Christian world, the current information technology revolution, particularly through mediums such as Facebook, Instagram, YouTube, and Twitter, has triggered the re-construction and decentralization of religious authority in Islam. New santri (pious individuals) and preachers emerged from the non-conventional religious educational system. They not only challenged the traditional authorities, but also redefine and re-conceptualize old religious terminologies, such as hijra and wasatiyya. This book explores the dynamics of religious authority in Indonesia with ...