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PCR Cloning Protocols
PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.
Fuzzy Systems and Knowledge Discovery
This book and its sister volume, LNAI 3613 and 3614, constitute the proce- ings of the Second International Conference on Fuzzy Systems and Knowledge Discovery (FSKD 2005), jointly held with the First International Conference on Natural Computation (ICNC 2005, LNCS 3610, 3611, and 3612) from - gust 27–29, 2005 in Changsha, Hunan, China. FSKD 2005 successfully attracted 1249 submissions from 32 countries/regions (the joint ICNC-FSKD 2005 received 3136 submissions). After rigorous reviews, 333 high-quality papers, i. e. , 206 long papers and 127 short papers, were included in the FSKD 2005 proceedings, r- resenting an acceptance rate of 26. 7%. The ICNC-FSKD 2005 conference featured the most...
International Symposium on Fuzzy Systems, Knowledge Discovery and Natural Computation (FSKD 2014)
ICNC-FSKD is a premier international forum for scientists and researchers to present the state of the art of data mining and intelligent methods inspired from nature, particularly biological, linguistic, and physical systems, with applications to computers, circuits, systems, control, communications, and more. This is an exciting and emerging interdisciplinary area in which a wide range of theory and methodologies are being investigated and developed to tackle complex and challenging problems.
Fuzzy Information and Engineering Volume 2
This book is the proceedings of the Third International Conference on Fuzzy Information and Engineering (ICFIE 2009) held in the famous mountain city Chongqing in Southwestern China, from September 26-29, 2009. Only high-quality papers are included. The ICFIE 2009, built on the success of previous conferences, the ICFIE 2007 (Guangzhou, China), is a major symposium for scientists, engineers and practitioners in the world to present their updated results, ideas, developments and applications in all areas of fuzzy information and engineering. It aims to strengthen relations between industry research laboratories and universities, and to create a primary symposium for world scientists in fuzzy fields as follows: Fuzzy Information; Fuzzy Sets and Systems; Soft Computing; Fuzzy Engineering; Fuzzy Operation Research and Management; Artificial Intelligence; Fuzzy Mathematics and Systems in Applications, etc.
Fuzzy Information and Engineering
This book is the proceedings of the Third Annual Conference on Fuzzy Information and Engineering (ACFIE2008) from Dec. 5-10, 2008 in Haikou, China. The conf- ence proceedingsis published by Springer-Verlag(Advancesin Soft Computing,ISSN: 1615-3871). This year, we have received 155 submissions. Each paper has undergone a rigorous review process. Only high-quality papers are included. The Third Annual Conference on Fuzzy Information and Engineering (ACFIE2008), built on the success of previous conferences,the ACFIE2005 (Guangzhou,China), is a major symposium for scientists, engineers and practitioners in China to present their updated results, ideas, devel- ments and applications in all areas ...
Antibody Phage Display
The closing years of the 19th century and the start of the 20th century witnessed the emergence of microbiology and immunology as discrete sci- tific disciplines, and in the work of Roux and Yersin, perhaps the first benefits of their synergy—immunotherapy against bacterial infection. As we advance into the new millennium, microbiology and immunology again offer a c- ceptual leap forward as antibody phage display gains increasing acceptance as the definitive technology for monoclonal production and unleashes new - portunities in immunotherapy, drug discovery, and functional genomics. In assembling Antibody Phage Display: Methods and Protocols, we have aimed to produce a resource of real va...
Genomic Imprinting
Genomic imprinting is the process by which gene activity is regulated according to parent of origin. Usually, this means that either the maternally inherited or the paternally inherited allele of a gene is expressed while the opposite allele is repressed. The phenomenon is largely restricted to mammals and flowering plants and was first recognized at the level of whole genomes. Nuclear transplantation experiments carried out in mice in the late 1970s established the non-equivalence of the maternal and paternal genomes in mammals, and a similar conclusion was drawn from studies of interploidy crosses of flowering plants that extend back to at least the 1930s. Further mouse genetic studies, involving animals carrying balanced translocations (reviewed in Chapter 3), indicated that imprinted genes were likely to be widely scattered and would form a minority within the mammalian genome. The first imprinted genes were identified in the early 1990s; over forty are now known in mammals and the list continues steadily to expand.
Biostatistical Methods
Leading biostatisticians and biomedical researchers describe many of the key techniques used to solve commonly occurring data analytic problems in molecular biology, and demonstrate how these methods can be used in the development of new markers for exposure to a risk factor or for disease outcomes. Major areas of application include microarray analysis, proteomic studies, image quantitation, genetic susceptibility and association, evaluation of new biomarkers, and power analysis and sample size.
Transgenesis Techniques
The past decade has witnessed a spectacular explosion in both the devel- ment and use of transgenic technologies. Not only have these been used to aid our fundamental understanding of biologic mechanisms, but they have also faci- tated the development of a range of disease models that are now truly beginning to impact upon our approach to human disease. Some of the most exciting model systems relate to neurodegenerative disease and cancer, where the availability of appropriate models is at last allowing radically new therapies to be developed and tested. This latter point is of particular significance given the current concerns of the wider public over both the use of animal models and the merits of using genetically modified organisms. Arguably, advances of the greatest significance have been made using mammalian systems—driven by the advent of embryonic stem-cell–based strategies and, more recently, by cloning through nuclear transfer. For this reason, this new edition of Transgenesis Techniques focuses much more heavily on manipulation of the mammalian genome, both in the general discussions and in the provision of specific protocols.
Telomeres and Telomerase
The fundamental problem that dividing cells have to ov- come is that of end-replication. Chromosomes shorten by many bases during DNA replication and so this presents a major hurdle that a cell has to overcome both to enable it to proliferate and for the larger organism to survive and reproduce. The enzyme telomerase provides a mechanism to ensure chromosome stability in both normal and neoplastic cells. The demonstration of telomerase expression in a majority of tumors and the realization of the potential role of telomerase in aging has opened up the potential for telomerase to be used as a target for therapeutic intervention. There is therefore great interest in the expression and activity of telomerase in a wide range of biological disciplines. Telomeres and Telomerase: Methods and Protocols has been produced as a tool for the many researchers in different areas of cell biology who are interested in following research in the area of telomerase and telomere maintenance, either in the area of fundamental mec- nisms or perhaps in the area of more applied drug discovery work.
