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The ability to highly purify and characterize hematopoietic stem cells (HSC) from mice and humans has opened up an exceedingly rich field of basic science research with enormous clinical potential. Many of the techniques used in st- ies of HSC biology have become more standardized over the last several years, which makes it possible to compile a set of methods that can be used by both seasoned investigators and novices in the stem cell field. We have attempted to be as comprehensive as possible and yet focus on what we perceive to be the most widely used approaches for studies of murine and human HSC. This first edition of Hematopoietic Stem Cell Protocols will therefore have some obvious om...
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Ages 14 and up.
These official Saab manuals are the only factory-authorized, comprehensive, single source of service information and specifications available. Whether you're a professional technician or a do-it-yourselfer, these manuals will help you understand, care for, and repair your Saab. Everything from fundamental automotive concepts and maintenance procedures to complex electrical system troubleshooting and complete engine overhaul is clearly explained. Critical updates and information from the Saab Service Information Manual, the Parts & Service Information System and Saab Service Training have been included, as well as fast, proven repair procedures and tips used by Saab technicians.
In these early 20th century literary essays, Stefan Zweig offers a Central European view of the writers he believed to be the “three greatest novelists” of the 19th century: Balzac, Dickens, and Dostoevsky. In Zweig’s view, Balzac set out to emulate his childhood hero Napoleon. Writing 20 hours a day, Balzac’s literary ambition was “tantamount to monomania in its persistence, its intensity, and its concentration.” His characters, each similarly driven by one desperate urge, were more vital to Balzac than people in his daily life. In Zweig’s reading, Dickens embodied Victorian England and its “bourgeois smugness”. His characters aspire to “A few hundred pounds a year, an a...
This is the second volume in a trilogy in which Stefan Zweig builds a composite picture of the European mind through intellectual portraits selected from among its most representative and influential figures. In Hölderlin, Kleist, and Nietzsche, Zweig concentrates on three giants of German literature to portray the artist and thinker as a figure possessed by a powerful inner vision at odds with the materialism and scientific positivism of his time, in this case, the nineteenth century. Zweig's subjects here are respectively a lyric poet, a dramatist and writer of novellas, and a philosopher. Each led an unstable life ending in madness and/or suicide and not until the twentieth century did...
Andrew Pollard and Martin Maiden have assembled an impressive collection of the latest molecular and cellular techniques for the development, evaluation, and implementation of vaccines to be used against this dreaded disease. The contributors-leading scientists, clinicians, and public health physicians-describe in detail the many approaches to vaccine design, as well as the assessment of immune response to vaccine candidates and novel vaccine formulations. Timely and comprehensive, Meningococcal Vaccines: Methods and Protocols provides the scientist, public health physician, epidemiologist, clinical microbiologist, and clinician with the essential tools to lay bare the secrets of the meningococcus, and to develop, evaluate, and implement successful new meningococcal vaccines.
Contents--Emerson; The Life of George Eliot; Daniel Deronda: A Conversation; Anthony Trollope; Robert Louis Stevenson; Miss Woolson; Alphonse Daudet; Guy de Maupassant; Ivan Turgenieff; George du Maurier; The Art of Fiction.