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Medical BioMethods Handbook
John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.
The Current Challenges Underlying Hepatitis D Virus Infection
The Human Hepatitis D virus (HDV) is one of the smallest human RNA viruses (22 nm), characterized by the peculiarity to require Hepatitis B virus (HBV) for its replication. Indeed, HDV utilizes HBV surface glycoprotein (HBsAg) for viral entry, assembly and release, implying the need for a intrahepatic transcriptionally active HBV to ensure HDV replication. Chronic HBV/HDV coinfection is associated with a high risk of developing liver cirrhosis and hepatocellular carcinoma within 5–10 years, resulting in high fatality rate. Recent estimates suggest that 9-60 million individuals may be infected with HDV worldwide. However, these fluctuating estimates highlight a huge uncertainty about the real prevalence of HDV infection, mostly related to the lack of robust data on large populations of HBsAg positive patients undergoing HDV screening. This highlights the need of accurate screening programs that finely trace the circulation of HDV.
Biobanks as Essential Tools for Translational Research: The Belgian Landscape
This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.
Carla and Antonio
“The sound of the church bells fills the air. I hurry along the cobblestone street listening to each peal and loving it. I breathe deeply as I pass one of the many chocolate shops and am rewarded with an enticing whiff of chocolate... The narrow streets are filled with people; many are tourists just like me. But I don’t feel like a tourist. I feel as if I once lived in this beautiful medieval city... I’m startled by a voice behind me. “And where are you going?” I whirl around. “Antonio! Don’t scare me like that!” He laughs and walks along with me. “How about stopping for a beer at that place along the canal?” Carla first meets Antonio in 1966 in Bruges, Belgium. They are each on a summer break from their American universities. Their friendship develops but complications begin when Patricia, a girl from England, arrives. Summer break draws to a close and Carla returns to Seattle to continue her studies and her life without really knowing what has become of Antonio. Will she ever hear from him again? Thirty years later...
My Mother's Delightful Deaths
A little girl's mom, who's an opera singer, plays with her and a neighbor child during the day. During the night, when she's on stage, a transformation occurs, and the death scenes leave a special impression on both the audience and her daughter.
More Than Words: Stories of Strength
They're your neighbors, your aunts, your sisters and your best friends. They're women across North America committed to changing and enriching lives, one good deed at a time. Three of these exceptional women have been selected as recipients of Harlequin's More Than Words award. And three New York Times bestselling authors have kindly offered their creativity to write original short stories inspired by these real-life heroines. We hope these stories inspired by strong, courageous women will touch your heart and motivate the heroine living inside you.
Carla
This book is a special thanks to some of the members of my church which has been a great inspiration to a lot of my writings. Sister Carla Boone/Hines, a mother, teacher and a friend, with her never ending organizations, positions, good deeds, and thoughtfulness reached out to me when I was slowing down and gave me inspirations. Brother Billie Broughton, Not only a brother in Christ, but like a brother in blood. He greets me and everyone when we get to church every Sunday morning. He is a husband,father and grandfather. He greets, cooks, serve, build, and help correct many mistakes that are mad by many helpers around the church. Sister Mary E. Coonce/Patterson. Who recently passed on was a wonderful,friend, Wife, Mother, Choir director, singer and council woman. Before passing on Sister Mary moved to Florida. Then there is Diamond Brand one of the most beautiful, young, God fearing ladies of this day and time and I am so happy to say that she is my niece, /love you DIAMOND
What Might Have Been Me
Carla Matthews travelled to New York as a student for a summer but when the time came to head home to Ireland, she decided to stay behind. She had fallen in love with musician boyfriend Eddie, with the city itself, with the idea that here she could become someone new, someone she couldn't be in Dublin anymore. Eleven years later, Carla feels stuck. She never did return to university and has almost forgotten her dream of being a writer. As she begins to wonder if this is how it will always be, she receives a phone call from home that changes everything. Now Carla must return to Dublin, to her mother and sister, to a city and a life she hardly recognises anymore. Faced with some difficult choices, Carla begins to discover what it truly means to come home to herself. What Might Have Been Me is a compelling story of love and belonging, and of how, in the midst of devastating loss, a family finds a way to piece itself back together.
