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Medical BioMethods Handbook
John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.
The Current Challenges Underlying Hepatitis D Virus Infection
The Human Hepatitis D virus (HDV) is one of the smallest human RNA viruses (22 nm), characterized by the peculiarity to require Hepatitis B virus (HBV) for its replication. Indeed, HDV utilizes HBV surface glycoprotein (HBsAg) for viral entry, assembly and release, implying the need for a intrahepatic transcriptionally active HBV to ensure HDV replication. Chronic HBV/HDV coinfection is associated with a high risk of developing liver cirrhosis and hepatocellular carcinoma within 5–10 years, resulting in high fatality rate. Recent estimates suggest that 9-60 million individuals may be infected with HDV worldwide. However, these fluctuating estimates highlight a huge uncertainty about the real prevalence of HDV infection, mostly related to the lack of robust data on large populations of HBsAg positive patients undergoing HDV screening. This highlights the need of accurate screening programs that finely trace the circulation of HDV.
Biobanks as Essential Tools for Translational Research: The Belgian Landscape
This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.
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