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Methods in Cell Biology
Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users.
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Flow Cytometry Protocols
Flow cytometry has evolved since the 1940s into a multidisciplinary field incorporating aspects of laser technology, fluid dynamics, electronics, optics, computer science, physics, chemistry, biology, and mathematics. Innovations in instrumentation, development of small lasers, discovery of new fluorochromes/fluorescent proteins, and implementation of novel methodologies have all contributed to the recent rapid expansion of flow cytometry applications. In this thoroughly revised and updated second edition of Flow Cytometry Protocols, time-proven as well as cutting-edge methods are clearly and comprehensively presented by leading experimentalists. In addition to being a valuable reference man...
Flow Cytometry
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Flow Cytometry, Part A
Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells.Key Features* Completely revised and greatly expanded since the publication of the First Edition in 1990* Methods cover cell death and cell cycle analysesPractical, handbook-style presentation works in lab or classroom* Unique comprehensive methodological coverage* Color plates illustrate techniques* In-depth treatment of procedures, including a description of each procedure:* Theoretical foundations* Critical aspects* Possible pitfalls* Written by authors with extensive experience who:* Developed or modified the techniques* Describe their experience with different instruments and applications to different cell systems* Are the Who's Who in Flow Cytometry
Essential Cytometry Methods
Cytometry is characterization and measurement of cells and cellular constituents, most often used to immunophenotype cells - that is, to distinguish healthy cells from diseased cells. Flow Cytometry specifically is quite sensitive, allowing researchers to detect rare cell types and residual levels of disease, and as such has been the method of choice for important studies such as monitoring the blood of AIDS patients. For this reason, there is a great need for a practical, comprehensive manual that will be useful across a broad range of laboratories. This volume, as part of the Reliable Lab Solution Series, delivers such a tool, offering busy researchers across many disciplines a handy resource of all the best methods and protocols for Cytometry to use at the bench. - Highlights top downloaded and cited chapters, authored by pioneers in the field and enhanced with their tips, and pitfalls to avoid - Loaded with detailed protocols developed and used by leaders in the field - Refines, organizes and updates popular methods from one of our top selling series, Methods in Cell Biology
