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Single-Domain Antibodies
This volume covers current and emerging techniques for studying single-domain antibodies (sdAbs). Chapters guide readers through the biology and immunology of sdAbs in camelids and sharks, isolation of sdAbs, protein engineering approaches to optimize the solubility, stability, valency and antigen binding affinity of sdAbs, and specialized applications of sdAbs. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Single-Domain Antibodies: Methods and Protocols aims to be a useful, practical guide to help researchers further their studies in this field.
The Girl Who Cried Too Much
THREE COLD CASES CLOSED With the closure of the oldest cold case in Paradise Hill, and the potential closure of two more, amateur sleuth and crime reporter Tess McClintock continues to work with FBI Special Agent Michael Carter. When doubts about the killer's identity arise, Tess and Michael to dig deeper into the cases, with help from members of The Missing - the internet sleuth group Tess belongs to. A KILLER ENJOYING THE MAYHEM While they get closer to the truth, Tess and Michael also grow closer, and their budding romance complicating the investigation. The real killer continues to target Tess, toying with her and Michael while he plots his next move. NEW EVIDENCE POINTS TO A NEW SUSPECT New evidence suggests another suspect may have been involved and police and FBI Agents rush to investigate, struggling to separate fact from fiction. THE GIRL WHO CRIED TOO MUCH, the second book in the McClintock-Carter Crime Thriller Series, follows Tess and Michael as they fight to stop a ruthless killer before he strikes again. THE ONLY GIRL LEFT ALIVE, is the third book and the conclusion to the series.
Board of Contract Appeals Decisions
The full texts of Armed Services and othr Boards of Contract Appeals decisions on contracts appeals.
The Snow Killings
Over 13 months in 1976-1977, four children were abducted in the Detroit suburbs, each of them held for days before their still-warm bodies were dumped in the snow near public roadsides. The Oakland County Child Murders spawned panic across southeast Michigan, triggering the most extensive manhunt in U.S. history. Yet after less than two years, the task force created to find the killer was shut down without naming a suspect. The case "went cold" for more than 30 years, until a chance discovery by one victim's family pointed to the son of a wealthy General Motors executive: Christopher Brian Busch, a convicted pedophile, was freed weeks before the fourth child disappeared. Veteran Detroit News reporter Marney Rich Keenan takes the reader inside the investigation of the still-unsolved murders--seen through the eyes of the lead detective in the case and the family who cracked it open--revealing evidence of a decades-long coverup of malfeasance and obstruction that denied justice for the victims.
