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Thirty-eight-year-old lawyer Audrey is tired of not being seen. Not seen by her mother, who always preferred her golden brother. By her sleazy boss, who works her to the bone, without reward or recognition. By her self-obsessed colleagues, who want her to help them fix their lives without any acknowledgement of her own. Her social life consists of late nights in the office, visits to her ageing parents, trivia nights with a group of relative strangers, and evenings at home with her pet rabbit Joni. One night, unable to get the attention of the bartender in her local, she walks out without paying for her wine. This small rebellion leads to another, and more. Liberated by her invisibility, Audrey wreaks havoc in the lives of her friends and workmates. Until a painful reminder from the past pushes her into a reckoning, and things really start to spiral out of control.
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This comprehensive and engaging Research Handbook provides a full examination of the modes and mechanisms of international knowledge transfer. Furthermore, it also provides in-depth insights into international knowledge transfer related challenges faced by multinational enterprises (MNEs).
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This detailed volume presents a series of protocols that are representative of recent developments and improvements in induced pluripotent stem cells (iPS cells) and corresponding human disease models. Reflecting the latest technology for generating induced pluripotent stem cells (iPS cells) and their initial characterization, the book explores techniques invaluable both for studies of disease-specific cell types and for their potential applications in regenerative medicine. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Induced Pluripotent Stem Cells and Human Disease: Methods and Protocols serves as a vital guide that is valuable for not only experts but also novices in the stem cell field.
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Charles Gilchrist was born in about 1775 in Scotland. His parents may have been John Gilchrist and Elizabeth Struthers. He married Catherine Robinson, daughter of Robert Robinson and Catherine, 16 August 1798 in Boston, Lincolnshire, England. They had seven children. Charles died in 1829. Descendants and relatives lived mainly in Lincolnshire, Leicestershire, Hampshire, Hertfordshire, Derbyshire and Dorset.