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Men's Health magazine contains daily tips and articles on fitness, nutrition, relationships, sex, career and lifestyle.
In Playing With Fire, Theo Fleury takes us behind the bench during his glorious days as an NHL player, and talks about growing up devastatingly poor and in chaos at home. Dark personal issues began to surface, and drinking, drugs, gambling, and girls ultimately derailed a career that had him destined for the Hall of Fame. Fleury shares all in this raw, captivating, and honest look at the previously untold story of one the game's greatest heroes.
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This special anniversary collection includes the 100 biggest accomplishments of American mountaineers, the most important voice in American climbing, the best books by American climbers and more. Climbers of 2001's hottest new routes includes Kenton Cool, Jonathan Copp, Stefan Glowacz, Alex and Tom Huber, Stephen Koch, Tim O'Neill, Dean Potter, Marko Preselj, Mark Richey, Raphael Slawinski, and more.
This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Surface Display serves as a comprehensive resource that enables the implementation of this powerful and versatile technique in virtually any molecular biology laboratory, even in the absence of any prior yeast surface display experience.