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This detailed book examines the main methods to study mammalian monoamine oxidases (MAOs), ranging from cell biology to computational chemistry. Beginning with techniques on how to obtain pure samples of MAO A and MAO B, the volume continues by covering assays and techniques used to measure MAO enzymatic activity and perform inhibition studies, methods to address cellular localization and function of MAOs, either in cell lines or in animal models, as well as computational methods applied to rational drug design approaches that are used to develop new MAO inhibitors. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Monoamine Oxidase: Methods and Protocols serves as a vital resource for scientists who are interested in studying MAOs and other similar amine oxidase enzymes.
This book provides essential information on a wide range of important issues in health sciences relating to child development, nutrition and dietetics, nursing, midwifery, and general health services. It also examines some issues and concerns in health management, including organizational trust in health care; artificial intelligence in healthcare, community-based rehabilitation in cerebral palsy; and digital marketing in the health sector. Contributions in each chapter are prepared by experts in the respective fields, and mirror advances in the respective field. This book sets out a number of important future tasks within the field, and supplies extensive bibliographies at the end of each chapter, as well as tables and figures that illustrate the research findings. All these make this book highly useful and a ‘must-read’ for students, researchers, and professionals in health sciences.
Structure and Dynamics of Macromolecules: Absorption and Fluorescence Studies is clearly written and contains invaluable examples, coupled with illustrations that demonstrate a comprehensible analysis and presentation of the data. This book offers practical information on the fundamentals of absorption and fluorescence, showing that it is possible to interpret the same result in different ways. It is an asset to students, professors and researchers wishing to discover or use absorption and fluorescence spectroscopy, and to scientists working on the structure and dynamics of macromolecules.* Offers concise information on the fundamentals of absorption and fluorescence * Critically reviews examples taken from previously published literature * Highly illustrated, it is suitable for academic and institutional libraries and government laboratories
Edited by one of the leading experts in the field, this book fills the need for a book presenting the most important methods for high-throughput screenings and functional characterization of enzymes. It adopts an interdisciplinary approach, making it indispensable for all those involved in this expanding field, and reflects the major advances made over the past few years. For biochemists, analytical, organic and catalytic chemists, and biotechnologists.
Optical Spectroscopy bridges a gap by providing a background on optics while focusing on spectroscopic methodologies, tools and instrumentations. The book introduces the most widely used steady-state and time-resolved spectroscopic techniques, makes comparisions between them, and provides the methodology for estimating the most important characteristics of the techniques such as sensitivity and time resolution. Recent developments in lasers, optics and electronics has had a significant impact on modern optical spectroscopic methods and instrumentations. Combining the newest lasers, advanced detectors and other high technology components researchers are able to assemble a spectroscopic instru...
Unique in providing an overview of the subject on the scientific level, this book presents the current state of the art with regard to different aspects of sustainable energy production and its efficient storage. The broad scope ranges from nanomaterials for energy production, via fuel cells and nanostructured materials for fuel production, right up to supercapacitors and climate change. Edited by a rising star within the community, this is an invaluable work on a hot topic for materials scientists, solid state, surface and physical chemists, as well as those chemists working in industry and chemical engineers.