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Apoptosis and Cancer
The aim of Apoptosis and Cancer is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. The protocols described will aid both the academic laboratory interested in further characterizing the mechanisms of apoptosis, as well as the industry laboratory, aimed at identifying new target molecules or screening for new compounds with potential clinical use.
The Ubiquitin Code
This volume details novel and popular approaches to decipher the Ubiquitin Code. Chapters guide readers through ubiquitin chain complexity, methods to generate non-hydrolysable ubiquitin-NEDD8, a fully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B, a HECT ligase-mediated ubiquitylation in vitro, a RNAi-based screening to identify DUBs, a Yuh1 activity assay, a pull-down to isolate K48 and K63 chains using specific nanobodies, identification of NeDDylated protein based in a NEDD8R74K mutant, in vivo identification of NEDDylated proteins from liver diseases, UbL-ID method to identify ubiquitin-like modified proteins, a LC3/GABARAP capturing system,...
Redox Biology in Plasma Medicine
Plasma medicine uses non-equilibrium plasmas generated under atmospheric-pressure conditions. Therapeutical plasmas can stimulate tissue regeneration or inactivate cancer cells. This book reviews the interrelation between plasma chemistry and biochemistry complemented by discussion of the ways plasmas inactivate various pathogens. Focus is on the plasma effects on mammalian cells, subsequent consequences for cell-biological processes, and plasma applicability specific medical therapies. Contributions illustrate the ways cold atmospheric-pressure plasma can be used as a controllable source of redox-active species and as a useful tool for research in redox biology. Key Features Summarizes plasma chemistry, biochemistry, and microbiology Documents the ways plasmas interact with lipids, membranes, and cells Reviews therapeutic uses of plasmas in medicine Focuses on uses of plasmas as cancer treatment
Regulators and Effectors of Small GTPases, Part E: GTPases Involved in Vesicular Traffic
Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport.This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.
Elah's Plaything
Can Love Be Born From Revenge? Self-made billionaire Elah Avakian has amassed power and influence beyond most people's wildest dreams. But all that matters is his lifelong quest for revenge against his hated enemy. After years of planning, Elah is finally ready to get the justice he seeks. Marrying her is the first step. Lottie Trufant knows her father is an honorable man, and she’ll do anything to protect him. Which is how she finds herself married to a stranger, a man who only sees her as a means to an end, a man she finds herself drawn to more and more each day…
From Dan to Beersheba and Beyond
From Dan to Beersheba and Beyond is a series of spiritual observations and opinions from an aging pastor on his first trip to Palestine. Traveling with a study group from Dallas Theological Seminary, this Maine pastor finally gets to experience the biblical places and times he has imagined since childhood and has studied and taught throughout his adult years. Pastor Blackstone shares insights and highlights from this thirty-year dream, joined by his daughter Marnie, the heroine of two previous books, Rendezvous in Paris and Though One Go with Me. Travel with this father-daughter team from the slopes of Mount Hermon in the north to the shores of the Red Sea in the south on this spectacular pi...
Physiological Consequences of Brain Insulin Action
The brain is crucial for the regulation of whole-body metabolism and behavior. The pancreas-derived hormone insulin modulates brain function and affects energy metabolism as well as cognition. This is partially achieved by modulating several types of brain cell populations including those relevant to satiety and reward. Importantly, brains of Alzheimer ́s disease patients exhibit a signature of brain insulin resistance with perturbed brain metabolism. This book will cover the basics of insulin signaling in the brain and will describe concepts of insulin resistance, a feature of type 2 diabetes. Moreover insulin ́s effect on cognitive function will be pointed out and the association between...
Cumulative Subject Index
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Supplements index volumes 33, 75, 95, 120, 140, 175, 199, 229, 265, 285, and 320 Subject index Contributor index
The Making of a King
Jonathan, King Sauls oldest son overheard the argument between his father and Samuel, the Prophet. The argument was fierce and tense. He had never seen Samuel so distraught. Samuel had just finished telling his father that he was no longer to be king of the country when Jonathan reached them. It must be a mistake, Jonathan thought. Samuel turned from Saul and ordered the two soldiers, Bring King Agag before me. In minutes, King Agag stood before them with a confidence that he would soon be free. He had been the only one spared from the attack of the city. Without hesitation Samuel turned and pulled the sword from King Sauls sheath and began to slay King Agag. After Samuel was through he retu...
