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Christoph Herwig is founder of Exputec GmbH.
This detailed volume presents a series of protocols dealing with different aspects of inclusion body (IB) processing, from cloning procedures to purification of refolded product. Commencing with chapters on upstream processing, looking into different expression strategies for IB production, the book continues with downstream applications, highlighting early protein purification and subsequent analytics, as well as success stories of IB-based processes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Inclusion Bodies: Methods and Protocols serves as an ideal resource for facilitating diverse aspects of IB processing.
Today, the agriculture industry is confronted with simultaneous issues of how to fully embrace mass production of safer food in terms of both quality and quantity. Most industries are concerned with avoiding significant levels of soil pollution and environmental threats as a result of the excessive and harmful use of synthetic products on crops. Therefore, there is a need to adopt sustainable technological innovations that can ensure the sustainability of agricultural production systems. Microbial Biostimulants for Sustainable Agriculture and Environmental Bioremediation discusses the benefits, challenges, and practical applications of eco-friendly biotechnological techniques using biostimul...
Biotechnology represents a major area of research focus, and many universities are developing academic programs in the field. This guide to biomanufacturing contains carefully selected articles from Wiley's Encyclopedia of Industrial Biotechnology, Bioprocess, Bioseparation, and Cell Technology as well as new articles (80 in all,) and features the same breadth and quality of coverage and clarity of presentation found in the original. For instructors, advanced students, and those involved in regulatory compliance, this two-volume desk reference offers an accessible and comprehensive resource.
The Society for the Advancement of Science in Africa (SASA) continues with its mission to advance science, improve health, and promote economic and social development on the African continent. It serves to push for continent-wide African innovation and new frontiers of scientific research. Its fifth annual conference was held jointly with the University of Rwanda under the theme “Translational Science and Biotechnology Advances in Africa”. This volume provides a selection of papers presented at the conference, encompassing diverse fields including biomedical sciences, health research, agricultural and soil sciences, advances in minimal invasive surgery, disease surveillance, pharmaceutical sciences, and genetics and genomics. The diversity of participants and spirited presentations covering over a dozen fields and sub-fields is indeed a true reflection of the tangible advancement of science in Africa.
Studying organisms as a whole for potential metabolic(ally) engineering of organisms for production of (bio)chemicals is essential for industrial biotechnology. To this end, integrative analysis of different –omics measurements (transciptomics, proteomics, metabolomics, fluxomics) provides invaluable information. Combination of experimental top-down and bottom-up approaches with powerful analytical tools/techniques and mathematical modeling, namely (quantitative) systems biology, currently making the state of art of this discipline, is the only practice that would improve our understanding for the purpose. The use of high-throughput technologies induced the required development of many bio...
Two strategies are usually considered for the optimization of microbial bioprocesses. The first one involves genetic or metabolic engineering of the target microbial strains in order to improve its production efficiency or its tolerance to adverse conditions. The second one is based on the chemical engineering improvement of the bioreactors and scaling-up rules. This work is more particularly dedicated to this second class of parameters. Recent developments in bioreactor technologies follow the scaling-out principle, i.e. carrying out several cultures in parallel with controlled conditions for screening purposes. Several mini-bioreactor concepts, i.e. bioreactor with working volume of 1 to 1...
In Biotechnology for Fuels and Chemicals: The Twenty-Ninth Symposium, leading US and international researchers from academia, industry, and government exchange cutting-edge technical information and update current trends in the development and application of biotechnology for sustainable production of fuels and chemicals. This symposium emphasizes advances in biotechnology to produce high-volume, low-price products from renewable resources, while improving the environment. The major areas of interest include advanced feedstock production and processing, enzymatic and microbial biocatalysis, bioprocess research and development, opportunities in biorefineries, and commercialization of biobased...