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Giardia and Cryptosporidium are both parasites of considerable global interest due to the gastrointestinal problems the organisms can cause in humans as well as domestic and wild animals. This book presents a comprehensive overview of recent research. Chapters discuss topics from taxonomy, nomenclature and evolution to molecular epidemiology, advances in diagnostics and zoonotic, human and animal health issues.
From the microscopic observation of infection to the widespread application of molecular techniques in taxonomy and epidemiology, to the genome sequencing of two major species and advances in biochemistry, phylogeny, and water treatment, new information on this fascinating genus continues to mount as we discover and utilize the latest scientific te
Cryptosporidium, in its various forms, is a widely recognised cause of outbreaks of waterborne disease. Regulatory bodies worldwide are increasingly requiring the development of "fit-for-purpose" detection methods for this protozoan parasite, but analysis is often problematic. Bringing together international academic and industry-based experts, this book provides a comprehensive review of the current state of analytical techniques for the detection of Cryptosporidium, as well as looking at likely future developments. In particular, the issues of species identification and oocyst viability are addressed. Quality assurance issues and potential problems associated with the new Cryptosporidium r...
Public Health Microbiology: Methods and Protocols is focused on microorganisms that can present a hazard to human health in the course of everyday life. There are chapters dealing with organisms that are directly pathogenic to humans, including bacteria, viruses, and fungi; on organisms that produce toxins during growth in their natural habitats; on the use of bacteriocins produced by such organisms as lactobacilli and bifidobacteria; as well as several chapters on hazard analysis, the use of disinfectants, microbiological analysis of cosmetics, and microbiological tests for sanitation equipment in food factories. Additional chapters look at the use of animals (mice) in the study of the vari...
Cryptosporidia and microsporidia are protozoan parasites that have emerged as human pathogens over the last two decades. With the coming of AIDS they have been recognised as important opportunistic pathogens of patients with an impaired host defence system. For both groups of pathogens, chemotherapeutic intervention is extremely limited. To date, cryptosporidiosis and microsporidiosis are severe and life-threatening opportunistic infections in AIDS patients and cryptosporidiosis is a major cause of diarrhea in infants and children in developing countries. This volume provides a comprehensive overview of the biology of the pathogens as well as the diagnosis and clinical aspects of the disease...
Due to the advent of AIDS, there is currently great interest in opportunistic infections in people with compromised immune systems. This special volume of Advances in Parasitology provides a detailed account ofthe pathogens associated with human enteric diseases, Cryptosporidium parvum, Enterocytozoon bieneusi and Cyclospora cayetanensis. This volume is essential for all parasitologists working on opportunistic protozoa.
This study focused on ideal methods of detecting C. parvum oocysts which give low detection limits, specific and sensitive detection in concentraded samples, and differentiation of viable from non-viable oocysts; are usable by a water treatment facility and provide quantitative results within a few hours. Potential useful methods are continuous flow centrifugation, rel-time TaqMan PCR, cell culture inoculation followed by PCR of DNA extration, and FISH.
This handbook includes basic information plus valuable resources to help troubleshoot and resolve problems by such organisms as Actinomycetes, Bloodworms, Crustacea, and more. Each section carries a brief description, the problems it can cause, control strategies and relevant references, color plates and more.