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The content covered encompass not only the theoretic and methodological aspects for culturing human pluripotent stem cells, but also the establishment of induced pluripotent stem cells and their differentiation into specific cellular subtypes. This book has the most updated methods and technologies in an accessible and clear language, presenting protocols developed and optimized by researchers that use them every day. Stem cell research is a growing field that offers the potential to comprehend mechanisms of neural development and tissue regeneration and maintenance as well as the development-related human diseases and the establishment of novel therapies. Questions of basic biology can also be exploited by this cutting-edge approach.
A obra que o leitor tem em mãos, fruto da tese de doutoramento do Professor Demétrius Coelho, que tive o privilégio de orientar no âmbito do Programa de Pós-Graduação em Direito da Faculdade de Direito da Uerj, busca – e alcança – a harmonia indispensável entre práxis e dogmática, tendo em vista as qualificações pessoais do autor, cujo cotidiano alia a intensa atividade docente na Pontifícia Universidade Católica do Paraná – Campus Londrina – à advocacia militante, com enorme vivência no foro. A desejada associação teoria-prática revela-se, com efeito, em todo o corpo da obra e, em especial, no tratamento dos conceitos jurídicos de adimplemento, autonomia privada e da própria cláusula resolutiva expressa, todos abordados segundo o método civil-constitucional, diga-se de passagem, a denotar a inserção acadêmica do autor na Uerj e a relação de pertinência temática entre o presente livro e os marcos teóricos da linha de pesquisa do direito civil no PPGD.
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This book highlights the current limitations of biogas production and yield and new avenues to improving them. Biogas production and yield are among the most important renewable energy targets for our world. Pursuing an innovative and biotechnological approach, the book presents alternative sources for biogas production and explores a broad range of aspects, including: pre-treatment of substrates, accelerators (enzyme-mediated) and inhibitors involved in the process of obtaining biogas and its yield, design specifications for digesters/modified digesters, managing biogas plants, microbial risk and slurry management, energy balance and positive climatic impacts of the biogas production chain, and the impacts on Human, Animal and Environmental Health (“One Health” concept for the biogas chain).
A ideia desta obra teve origem com a publicação da Lei n.o 13.467/17, que acrescentou o § 3o ao art. 8o da Consolidação das Leis do Trabalho (CLT). Esse novo comando normativo impôs limites à atuação da Justiça do Trabalho no momento do exame de convenção coletiva ou acordo coletivo, melhor explicando, limitou a análise desses instrumentos jurídicos negociados aos requisitos de validade do negócio jurídico, estabelecidos pelo art. 104 do Código Civil. Até um novo princípio, denominado princípio da intervenção mínima na autonomia da vontade coletiva, foi criado pelo legislador com evidente intuito de firmar a delimitação da atuação da Justiça do Trabalho. E com o o...
This book fills a gap in international literature by providing critical reviews on variables of organizational behavior and the main psychological instruments developed to measure them. Measuring instruments developed with theoretical and methodological rigor in the field of Organizational and Work Psychology can contribute to the development of diagnostic analyses to enable organizations to implement the evidence-based changes required for their survival. These changes demand diagnoses based on precise assessments of organizational and individual variables, but many times the professionals responsible for conducting these assessments are not sure of what is the best measuring instrument ava...