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In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
The International Corporate 1000 represents a joint venture between Monitor Publishing Com pany of Washington, DC, and Graham & Trotman Limited of London. Monitor Publishing Company is well known as the publisher of The Federal Yellow Book, The Congressional Yellow Book, and The Corporate 1000. Graham & Trotman's annual directories providing data on the major companies in many parts of the world are equally established. The two publishing companies have pooled their expertise in this joint venture to research, compile and publish The International Corporate 1000, A Directory o/Who Runs The World's 1000 Leading Corporations. The directory was designed to help you identify and contact the seni...
Our images of non-Western cultures are often based on stereotypes that are replicated over the years. These stereotypes often appear in popular media and are responsible for a pre-set image of otherness. The present book investigates these processes and the media representation of otherness, especially as an artificial construct based on stereotypes and their repetition, in the case of Japan. 'Western Japaneseness' thereby illustrates how the Western image of Japan in popular media is rather a construct that, in a way, replicated itself, instead of a more serious encounter with a foreign and different cultural context. This book will be of great value to students and academics who hold interest in media studies, Japanese studies, and cultural studies. It will also appeal to a broader audience with interests in Japan more generally.
This Research Topic is part of the Insights in Frontiers in Cardiovascular Medicine series. Following the success of the inaugural series, Frontiers has organized a series of Research Topics to highlight the latest advancements in research across the field of cardiovascular medicine, with articles from the members of our accomplished Editorial Boards. This editorial initiative of particular relevance, led by Professor Masanori Aikawa, Specialty Chief Editor of the Atherosclerosis and Vascular Medicine section, is focused on new insights, novel developments, current challenges, latest discoveries, recent advances, and future perspectives in the field of atherosclerosis.
pt. 1. List of patentees.--pt. 2. Index to subjects of inventions.
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