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Stem Cells in the Lung
This book presents a comprehensive overview of the current understanding of the organization of endogenous lung stem and progenitor cell compartments during fetal lung development, postnatal lung growth and in adulthood. Progressing stage by stage, the chapters on fetal lung development emphasize the integrated role of epithelial, stromal, vascular and neural cell elements in building a functional lung, while the subsequent chapters on adult lung regeneration describe the nature and properties of adult lung stem/progenitor cells distributed along the proximal-distal axis of the airway tree. The chapters on regulation of lung regeneration and repair discuss how regenerative cells interact wit...
Flow Cytometry Protocols
Flow cytometry has evolved since the 1940s into a multidisciplinary field incorporating aspects of laser technology, fluid dynamics, electronics, optics, computer science, physics, chemistry, biology, and mathematics. Innovations in instrumentation, development of small lasers, discovery of new fluorochromes/fluorescent proteins, and implementation of novel methodologies have all contributed to the recent rapid expansion of flow cytometry applications. In this thoroughly revised and updated second edition of Flow Cytometry Protocols, time-proven as well as cutting-edge methods are clearly and comprehensively presented by leading experimentalists. In addition to being a valuable reference man...
Hematopoietic Stem Cell Protocols
The ability to highly purify and characterize hematopoietic stem cells (HSC) from mice and humans has opened up an exceedingly rich field of basic science research with enormous clinical potential. Many of the techniques used in st- ies of HSC biology have become more standardized over the last several years, which makes it possible to compile a set of methods that can be used by both seasoned investigators and novices in the stem cell field. We have attempted to be as comprehensive as possible and yet focus on what we perceive to be the most widely used approaches for studies of murine and human HSC. This first edition of Hematopoietic Stem Cell Protocols will therefore have some obvious om...
Gene Knockout Protocols
As the major task of sequencing the human genome is near completion and full complement of human genes are catalogued, attention will be focused on the ultimate goal: to understand the normal biological functions of these genes, and how alterations lead to disease states. In this task there is a severe limitation in working with human material, but the mouse has been adopted as the favored animal model because of the available genetic resources and the highly conserved gene conservation linkage organization. In just of ten years since the first gene-targeting experiments were p- formed in embryonic stem (ES) cells and mutations transmitted through the mouse germline, more than a thousand mou...
Colony-Stimulating Factors
Concentrating on proven data and adopting a structure-function approach, this text provides grounding for an intricate understanding of the molecular biology, physiological mechanisms, and routine clinical use in disease settings of colony-stimulating factors (CSFs). This edition includes eight additional chapters, with updates of recently-discovered and established CSFs, each indexed individually.
Stem Cells and Cancer Stem Cells, Volume 12
Volume 12 in this series explores the latest experimental and clinical uses of stem cells in the treatment of disease and of injuries and reviews methods for isolating multipotent endothelial-like cells from human adipose tissue and discusses clinical applications in cell therapy and regenerative medicine. The book is organized in five parts: Cancer Stem Cells, Pluripotent Stem Cells, Dendritic Stem Cells, Regenerative Medicine and General Applications. The first section includes chapters on histamine in the neural and cancer stem cell niches and emerging concepts of stem cell organization in the normal lung and in lung cancer. The section on Pluripotent Stem Cells includes discussion of the...
Basic Cell Culture Protocols
Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.
