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Monoclonal Antibodies
Monoclonal Antibodies: A Practical Approach covers the preparation, testing, derivation, and applications of monoclonal antibodies. New immunological techniques incorporating tried and tested methodologies are described, making the book of interest to established and inexperienced immunologists. Both the standard somatic hybridization technique and recombinant techniques, including the use of phage libraries, for the preparation of rodent and human monoclonal antibodies are described. Protocols for both the small and large scale production are detailed, as well as purification and labelling (with both radioisotopes and non-radioisotopes) methods. The applications of monoclonal antibodies in immunoblotting, enzyme linked immunoassays, immunofluorescence, and FACS analysis are all covered in detail. Finally protocols are given for the use of monoclonal antibodies in rheumatoid arthritis, tissue typing, detecting DNA modified during chemotherapy, and in the clinical analysis of transplantation samples for malignancy. This book will therefore be an invaluable laboratory companion to anyone using monoclonal antibodies in their research.
Consistent Quantum Theory
Quantum mechanics is one of the most fundamental yet difficult subjects in physics. Nonrelativistic quantum theory is presented here in a clear and systematic fashion, integrating Born's probabilistic interpretation with Schrödinger dynamics. Basic quantum principles are illustrated with simple examples requiring no mathematics beyond linear algebra and elementary probability theory. The quantum measurement process is consistently analyzed using fundamental quantum principles without referring to measurement. These same principles are used to resolve several of the paradoxes that have long perplexed physicists, including the double slit and Schrödinger's cat. The consistent histories formalism used here was first introduced by the author, and extended by M. Gell-Mann, J. Hartle and R. Omnès. Essential for researchers yet accessible to advanced undergraduate students in physics, chemistry, mathematics, and computer science, this book is supplementary to standard textbooks. It will also be of interest to physicists and philosophers working on the foundations of quantum mechanics.
Fundamental and Applied Aspects of Animal Cell Cultivation
The advent of modern, biological techniques such as hybridoma technology, recombinant DNA techniques and viral transformation of cells has made the continuous production of a wide variety of biologicals possible using animal cells. The use of such products is well established in many diagnostic and (increasingly) therapeutic applications - the U.S. market for antibodies, for example, has been projected to increase from a 1991 level of US$0.33 billion to 1998 level of US$3.8 billion. Total sales of such products in 1992 was US$4.2 billion. The increasing application of this technology depends on increasing the efficiency of production and bioseparation and addressing various safety issues. This book examines the fundamental and applied aspects of animal cell cultivation.
Cell Culture Technology for Pharmaceutical and Cell-Based Therapies
Edited by two of the most distinguished pioneers in genetic manipulation and bioprocess technology, this bestselling reference presents a comprehensive overview of current cell culture technology used in the pharmaceutical industry. Contributions from several leading researchers showcase the importance of gene discovery and genomic technology devel
Large-Scale Mammalian Cell Culture Technology
An interdisciplinary approach, integrating biochemistry, biology, genetics, and engineering for the effective production of protein pharmaceuticals. The volume offers a biological perspective of large-scale animal cell culture and examines diverse processing strategies, process management, regulator
Gravitation, Following the Prague Inspiration
This is a festschrift celebrating the 60th birthday of Professor Jiri Bicak. The contributors are his former students currently working in the fields of general relativity, astrophysics, theoretical physics and cosmology. The articles present original results or survey those already published elsewhere. The subjects range from the motion of stars in galactic nuclei to quantum mechanics on a boundary, and include several hot topics of relativistic physics -- cosmological perturbations, the repulsive cosmological constant, discs around black holes, and gravitational waves.
A Very Greedy Drug
First Published in 1997. This book sketches the recent history of the panic over cocaine in Britain, before reporting in-depth research on more than a hundred users in Scotland. This group contained all types of users, from casual users to heavy users who had cut down without problems, to people with multiple drug problems. The book considers why some people can give up cocaine and others become addicted. It compares British users with those elsewhere in the world, showing that the effects are similar everywhere -- cocaine does not addict most of its users. The picture of drug use obtained from addicts in clinics is a distorted one. Cocaine use in Britain has caused much concern but its extent has been unknown. Users are still quite affluent, but when cocaine hits street level -- as it is predicted to do in the late 1990s -- problems are likely to increase.
Upstream Industrial Biotechnology, 2 Volume Set
Biotechnology represents a major area of research focus, and many universities are developing academic programs in the field. This guide to biomanufacturing contains carefully selected articles from Wiley's Encyclopedia of Industrial Biotechnology, Bioprocess, Bioseparation, and Cell Technology as well as new articles (80 in all,) and features the same breadth and quality of coverage and clarity of presentation found in the original. For instructors, advanced students, and those involved in regulatory compliance, this two-volume desk reference offers an accessible and comprehensive resource.
Safety in Cell and Tissue Culture
It is now more than half a century since animal cells first came into regular use in the laboratory. Instances of laboratory acquired infection and con tamination of therapeutic products, derived from the use of animal cell cultures are rare. The use of animal cells, in addition to an established role in the production of vaccines and therapeutic proteins, has many new medical applications including gene therapy, tissue engineering and cell therapy. Furthermore, C;ldvances in molecular and cell biology are enabling rapid development and application of these technologies and the development of new and more sensitive methods, such as nucleic acid amplification, for the characterisation of cell...
Bioreactor System Design
Describes the state-of-the-art techniques and methods involved in the design, operation, preparation and containment of bioreactor systems, taking into account the interrelated effects of variables associated with both upstream and downstream stages of the design process. The importance of the initial steps in the development of a bioprocess, such as strain and media selection, that have an overwhelming influence on all further operations, is emphasized.;This work is intended for biochemical, chemical and bioprocess engineers; biotechnologists; industrial biochemists; micro- and molecular biologists; food scientists; and upper-level undergraduate and graduate students in these disciplines.
