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Monoclonal Antibodies
Monoclonal Antibodies: A Practical Approach covers the preparation, testing, derivation, and applications of monoclonal antibodies. New immunological techniques incorporating tried and tested methodologies are described, making the book of interest to established and inexperienced immunologists. Both the standard somatic hybridization technique and recombinant techniques, including the use of phage libraries, for the preparation of rodent and human monoclonal antibodies are described. Protocols for both the small and large scale production are detailed, as well as purification and labelling (with both radioisotopes and non-radioisotopes) methods. The applications of monoclonal antibodies in immunoblotting, enzyme linked immunoassays, immunofluorescence, and FACS analysis are all covered in detail. Finally protocols are given for the use of monoclonal antibodies in rheumatoid arthritis, tissue typing, detecting DNA modified during chemotherapy, and in the clinical analysis of transplantation samples for malignancy. This book will therefore be an invaluable laboratory companion to anyone using monoclonal antibodies in their research.
Consistent Quantum Theory
Quantum mechanics is one of the most fundamental yet difficult subjects in physics. Nonrelativistic quantum theory is presented here in a clear and systematic fashion, integrating Born's probabilistic interpretation with Schrödinger dynamics. Basic quantum principles are illustrated with simple examples requiring no mathematics beyond linear algebra and elementary probability theory. The quantum measurement process is consistently analyzed using fundamental quantum principles without referring to measurement. These same principles are used to resolve several of the paradoxes that have long perplexed physicists, including the double slit and Schrödinger's cat. The consistent histories formalism used here was first introduced by the author, and extended by M. Gell-Mann, J. Hartle and R. Omnès. Essential for researchers yet accessible to advanced undergraduate students in physics, chemistry, mathematics, and computer science, this book is supplementary to standard textbooks. It will also be of interest to physicists and philosophers working on the foundations of quantum mechanics.
Fundamental and Applied Aspects of Animal Cell Cultivation
The advent of modern, biological techniques such as hybridoma technology, recombinant DNA techniques and viral transformation of cells has made the continuous production of a wide variety of biologicals possible using animal cells. The use of such products is well established in many diagnostic and (increasingly) therapeutic applications - the U.S. market for antibodies, for example, has been projected to increase from a 1991 level of US$0.33 billion to 1998 level of US$3.8 billion. Total sales of such products in 1992 was US$4.2 billion. The increasing application of this technology depends on increasing the efficiency of production and bioseparation and addressing various safety issues. This book examines the fundamental and applied aspects of animal cell cultivation.
Cell Culture Technology for Pharmaceutical and Cell-Based Therapies
Edited by two of the most distinguished pioneers in genetic manipulation and bioprocess technology, this bestselling reference presents a comprehensive overview of current cell culture technology used in the pharmaceutical industry. Contributions from several leading researchers showcase the importance of gene discovery and genomic technology devel
Exact Space-Times in Einstein's General Relativity
Einstein's theory of general relativity is a theory of gravity and, as in the earlier Newtonian theory, much can be learnt about the character of gravitation and its effects by investigating particular idealised examples. This book describes the basic solutions of Einstein's equations with a particular emphasis on what they mean, both geometrically and physically. Concepts such as big bang and big crunch-types of singularities, different kinds of horizons and gravitational waves, are described in the context of the particular space-times in which they naturally arise. These notions are initially introduced using the most simple and symmetric cases. Various important coordinate forms of each solution are presented, thus enabling the global structure of the corresponding space-time and its other properties to be analysed. The book is an invaluable resource both for graduate students and academic researchers working in gravitational physics.
Upstream Industrial Biotechnology, 2 Volume Set
Biotechnology represents a major area of research focus, and many universities are developing academic programs in the field. This guide to biomanufacturing contains carefully selected articles from Wiley's Encyclopedia of Industrial Biotechnology, Bioprocess, Bioseparation, and Cell Technology as well as new articles (80 in all,) and features the same breadth and quality of coverage and clarity of presentation found in the original. For instructors, advanced students, and those involved in regulatory compliance, this two-volume desk reference offers an accessible and comprehensive resource.
Introduction to Quantum Mechanics
Changes and additions to the new edition of this classic textbook include a new chapter on symmetries, new problems and examples, improved explanations, more numerical problems to be worked on a computer, new applications to solid state physics, and consolidated treatment of time-dependent potentials.
A Very Greedy Drug
First Published in 1997. This book sketches the recent history of the panic over cocaine in Britain, before reporting in-depth research on more than a hundred users in Scotland. This group contained all types of users, from casual users to heavy users who had cut down without problems, to people with multiple drug problems. The book considers why some people can give up cocaine and others become addicted. It compares British users with those elsewhere in the world, showing that the effects are similar everywhere -- cocaine does not addict most of its users. The picture of drug use obtained from addicts in clinics is a distorted one. Cocaine use in Britain has caused much concern but its extent has been unknown. Users are still quite affluent, but when cocaine hits street level -- as it is predicted to do in the late 1990s -- problems are likely to increase.
The Theory of Classical Dynamics
This textbook describes in detail the classical theory of dynamics, a subject fundamental to the physical sciences, which has a large number of important applications. The author's aim is to describe the essential content of the theory, the general way in which it is used, and the basic concepts that are involved. No deep understanding can be obtained simply by examining theoretical considerations, so Dr Griffiths has included throughout many examples and exercises. This then is an ideal textbook for an undergraduate course for physicists or mathematicians who are familiar with vector analysis.
Advanced Biotechnology
Biotechnology is the application of biological agents in either manufacturing industry or service operation. The essence of biotechnology is its multi-disciplinary nature requiring wide range of science and engineering inputs. The ultimate success of biotechnology is dependent upon advances in and support for the fundamental sciences which form its substratum. In this book, some important features of microbiology, biochemistry, genetics, and engineering which have a significant bearing on the education and development of biotechnology are highlighted. This book may stimulate the application of scientific and engineering principles to the processing of materials by biological agents in the service of the most urgent human needs.
