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DNA Microarrays, Part A: Array Platforms and Wet-Bench Protocols
Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results. Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshoot...
Care of the Critically Ill Patient
During the past decade there has been a considerable expansion in the understanding, assessment and treatment of critically ill patients. An attempt to portray our present knowledge of this diverse field in a comprehensive textbook is therefore a formidable and daunting undertaking. However, many colleagues in different disciplines and from different countries convinced us of the need for such a work and, greatly encouraged by their interest, we embarked upon the project. Any single text, even a very long one, cannot cover every aspect of critical illness. Our first task was, therefore, to define those areas of the subject that had to be included. This we did on a systematic basis, and altho...
Legislative Documents
Contains the reports of state departments and officials for the preceding fiscal biennium.
Oceanographic Processes of Coral Reefs
In the last two decades since publication of the first edition, substantial advancements have been made in the science, the need for transdisciplinary approaches to coral reef protection greater than ever before. This new edition, now in full color throughout with accompanying animations, goes beyond identifying foundational information and current problems to pinpoint science-based solutions for managers, stakeholders and policy makers. Coral reefs are connected by currents that carry plankton and the larvae of many reef-based organisms. Further, they supply food to reefs. Currents also bring pollutants from the land and, together with the atmosphere, affect the surrounding ocean. The chapt...
Genetic Engineering of Crop Plants
Genetic Engineering of Crop Plants is a proceeding of The 49th Nottingham Easter School in Agricultural Science, which was held at Sutton Bonington on April 17-21, 1989. This symposium discussed progress in the generation of crop species resistant to herbicides, viruses, and insects. The book discusses topics such as the genetic manipulation in plants; genetic engineering of crops for insect and herbicide resistance; the expression of heat shock gene in transgenic plants; and tuber-specific gene expression. The book also covers topics such as regulation of gene expression in transgenic tomato plants; the molecular biology of pea seed development; and the regulatory elements of maize storage protein genes. The text is recommended for experts in the field of botany, agriculture, and genetics who would like to know more about the improvement of crop plants through genetics.
Practical Fracture Mechanics in Design
Theoretical treatments of fracture mechanics abound in the literature. Among the first books to address this vital topic from an applied standpoint was the first edition of Practical Fracture Mechanics in Design. Completely updated and expanded to reflect recent developments in the field, the second edition of this valuable reference concisely revi
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