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Protein Purification
This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.
Chromatography
Leading researchers discuss the past and present of chromatography More than one hundred years after Mikhail Tswett pioneered adsorption chromatography, his separation technique has developed into an important branch of scientific study. Providing a full portrait of the discipline, Chromatography: A Science of Discovery bridges the gap between early, twentieth-century chromatography and the cutting edge of today’s research. Featuring contributions from more than fifty award-winning chromatographers, Chromatography offers a multifaceted look at the development and maturation of this field into its current state, as well as its importance across various scientific endeavors. The coverage inc...
Microspheres and Microcapsules in Biotechnology
Microspheres and microcapsules have very broad applications in various fields, especially in those of biotechnology and biopharmaceuticals, as targeting drug–delivery carriers, separation media for protein, peptide, DNA, and so forth. It is a big challenge to design and prepare microspheres and microcapsules of different sizes and structures from various materials and develop new techniques. This book focuses on new microspheres and microcapsules specifically designed and prepared for application in the fields of biotechnology and biopharmaceuticals involving bioreaction, bioseparation, bioformulation, biodetection, and other new bioapplications. It provides a deep knowledge about the principles of design, preparation methods, and application results of new microspheres and microcapsules for each bioapplication area. The book also presents problems that need to be studied further and comments on the future prospects of microspheres and microcapsules.
Mixed-Mode Chromatography
The book is about the technology and application of Mixed-mode chromatography (MMC). Unlike conventional single-mode HPLC, which resolves the analytes primarily based on their ionic or hydrophobic properties, MMC employs multifunctional stationary phases to exploit at least two modes of interactions (i.e., ionic and hydrophobic) with the analytes and as such often provides resolution that far exceeds that observed with a single-mode process. Over the past two decades, MMC has developed into an important analytical and purification tool in a number of applications in pharmaceutical and biotechnology industries. The technique has been used widely for the analyses of nucleic acids, amino acids, peptides, proteins, glycoproteins, carbohydrates, antibiotics, vaccines, and other products. The purpose of this book is to present a comprehensive survey of mixed-mode chromatography and is intended as a reference guide for graduate students and experienced scientists in pharmaceutical and biotechnology disciplines wishing to gain a deep understanding of this continuously evolving technology.
Protein Purification Process Engineering
Offers coverage of the development of protein purification processes for large-scale commercial operations, and addresses process development, scale-up, applications and mathematical descriptions. Technologies currently used at the commercial scale are covered in depth.
Fundamentals and Techniques
Fundamentals and Techniques
Applications
Applications
