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Specialised membrane domains of plasmodesmata, plant intercellular nanopores
Plasmodesmata (PD) are plant-specific intercellular nanopores defined by specialised domains of the plasma membrane (PM) and the endoplasmic reticulum (ER), both of which contain unique proteins, and probably different lipid compositions than the surrounding bulk membranes. The PD membranes form concentric tubules with a minimal outer diameter of only 50 nm, and the central ER strand constricted to ~10-15 nm, representing one of the narrowest stable membrane tubules in nature. This unique membrane architecture poses many biophysical, structural and functional questions. PM continuity across PD raises the question as to how a locally confined membrane site is established and maintained at PD....
The Plant Cytoskeleton
This detailed volume explores the development of technologies and protocols that are currently being used to understand the nature and activities of the plant cytoskeleton. A focus for many of the chapters is on sample preparation, as the quality of plant organ/tissue preparation, from single to multicellular samples, determines the quality of the data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Plant Cytoskeleton: Methods and Protocols serves as an ideal guide for researchers interested in or starting to be interested in plant cell and molecular biology research.
Science
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Biotechnology
Over the recent years, biotechnology has become responsible for explaining interactions of biological tools and processes so that many scientists in the life sciences from agronomy to medicine are engaged in biotechnological research. This book contains an overview focusing on the research area of molecular biology, molecular aspects of biotechnology, synthetic biology and agricultural applications in relevant approaches. The book deals with basic issues and some of the recent developments in biotechnological applications. Particular emphasis is devoted to both theoretical and experimental aspect of modern biotechnology. The primary target audience for the book includes students, researchers...
The Stasi Game
The Stasi and MI6 clash in this gripping Cold War crime thriller, from the award-winning author of Stasi Child. ______________________ A man's body is found buried in concrete at a building site in the new town district. When People's Police homicide captain Karin Müller arrives at the scene, she discovers that all of the body's identifiable features have been removed - including its fingertips. The deeper Müller digs, the more the Stasi begin to hamper her investigations. She soon realises that this crime is just one part of a clandestine battle between two secret services - the Stasi of East Germany and Britain's MI6 - to control the truth behind one of the deadliest events of World War II. The Stasi Game brilliantly fictionalises the true story of how Britain's wartime leaders justified the fire-bombing of German city of Dresden, which many have since condemned as a war crime.
The People's Game
From star players to rioting fans, The People's Game examines how football shaped the history of communist East Germany.
RNA Tagging
This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Tagging: Methods and Protocols serves as a vital reference for researchers looking to further the increasingly important research in RNA biology.
