Welcome to our book review site go-pdf.online!
You may have to Search all our reviewed books and magazines, click the sign up button below to create a free account.
Glutathione Transferases and Gamma-Glutamyl Transpeptidases
Focuses on particular aspects of the so-called Phase II of drug detoxication, which has important ramifications for endogenous metabolism and nutrition. This volume on glutathione transferases and gamma-glutamyl transpeptidases serves to bring together methods and concepts in a rapidly developing field of cell and systems biology.
Glycomics
In the past decade, there has been an explosion of progress in understanding the roles of carbohydrates in biological systems. This explosive progress was made with the efforts in determining the roles of carbohydrates in immunology, neurobiology and many other disciplines, examining each unique system and employing new technology. This volume represents the second of three in the Methods in Enzymology series, including Glycobiology (vol. 415) and Functional Glycomics (vol. 417), dedicated to disseminating information on methods in determining the biological roles of carbohydrates. These books are designed to provide an introduction of new methods to a large variety of readers who would like to participate in and contribute to the advancement of glycobiology. The methods covered include structural analysis of carbohydrates, biological and chemical synthesis of carbohydrates, expression and determination of ligands for carbohydrate-binding proteins, gene expression profiling including micro array, and generation of gene knockout mice and their phenotype analyses.
Regulators of G Protein Signaling, Part A
Regulators of G Protein Signaling, Part A is an in-depth treatment of G-Protein Signaling, and will cover general methods of analysis of RGS protein analysis, including Expression and post-translational modification, Assays of GAP activity and allosteric control, Electrophysiological methods and RGS-insensitive Ga subunits, Mouse models of RGS protein action, Methods of RGS protein inhibition, and G-protein regulators of model organisms. - Table of Contents - Expression and post-translational modification - Assays of GAP activity and allosteric control - Electrophysiological methods and RGS-insenstitive Ga subunits - Mouse Models of RGS protein action - Methods of RGS protein inhibition - G-protein regulators of model organisms
Energetics of Biological Macromolecules, Part D
This volume focuses on the cooperative binding aspects of energetics in biological macromolecules. Methodologies such as NMR, small-angle scattering techniques for analysis, calorimetric analysis, fluorescence quenching, and time resolved FRET measurements are discussed.*Methods for Evaluating Cooperativity in a Dimeric Hemoglobin*Multiple-Binding of Ligands to a Linear Biopolymer*Fluorescence Quenching Methods to Study Protein-Nucleic Acid Interactions*Linked Equilibria in Biotin Repressor Function: Thermodynamic, Structural and Kinetic Analysis
Oxygen Sensing
The ability of cells to sense and respond to changes in oxygenation underlies a multitude of developmental, physiological, and pathological processes. This volume provides a comprehensive compendium of experimental approaches to the study of oxygen sensing in 48 chapters that are written by leaders in their fields.
RNA Polymerase and Associated Factors, Part C
RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein.* Construction and purification of RNA polymerases* DNA microarrays and bacterial gene expression* Functional analysis of transcription factors
DNA Repair, Part A
DNA Repair, Part A provides detailed coverage of modern methods for molecular analysis of enzymes and enzyme systems that function in the maintenance of genome integrity. Coverage areas include base excision repair, nucleotide excision repair, translesion DNA polymerases, mismatch repair, genetic recombination, and double strand break repair. - A laboratory standard for more than 40 years - Over 400 volumes strong - Also available on ScienceDirect - Part A of a 2-part series
DNA Microarrays, Part A: Array Platforms and Wet-Bench Protocols
Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results. Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshoot...
DNA Microarrays, Part B: Databases and Statistics
Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results. Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshoot...
Glycobiology
In the past decade, there has been an explosion of progress in understanding the roles of carbohydrates in biological systems. This explosive progress was made with the efforts in determining the roles of carbohydrates in immunology, neurobiology and many other disciplines, examining each unique system and employing new technology. This volume represents the first of three in the Methods in Enzymology series, including Glycomics (vol. 416) and Functional Glycomics (vol. 417), dedicated to disseminating information on methods in determining the biological roles of carbohydrates. These books are designed to provide an introduction of new methods to a large variety of readers who would like to participate in and contribute to the advancement of glycobiology. The methods covered include structural analysis of carbohydrates, biological and chemical synthesis of carbohydrates, expression and determination of ligands for carbohydrate-binding proteins, gene expression profiling including micro array, and generation of gene knockout mice and their phenotype analyses.
