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Acclaimed as the most practical guide to plant tissue culture, the book is now even better and introduces new developments in biotechnology, such as genetic engineering and cell culture.
This fully revised fourth edition features background information and instructions for growing plants from cell structure and tissue culture and is written in terms that can be easily understood by both hobby botanists and experienced commercial growers.
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Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.
Thirty years ago, in vitro propagation was a new technique for producing plants, and Lydiane Kyte’s Plants from Test Tubes became the standard work on the topic. The new fourth edition has been thoroughly revised and updated to reflect the many advances in science and technology, including the five accepted sequential stages of micropropagation. Ten new plants have been added. This in turn has greatly expanded the already extensive bibliography. Among the new topics that have been introduced or expanded on are embryo culture for breeding, somaclonal variation, anther culture, somatic embryogenesis, cryopreservation, and genetic engineering. More ornamental plant examples are given and many new illustrations provided, including a chronology of discoveries in micropropagation.
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A guide to propagation from the author of The New Shade Garden, with over 500 photographs: “My bible for rejuvenating plants.” —Anne Raver, The New York Times For people who love gardens, propagation—the practice of growing whatever you want, whenever you want—is gardening itself. In Making More Plants, one of America's foremost gardening authorities, presents innovative, practical techniques for expanding any plant collection, along with more than 500 photographs. Based on years of research, this is a practical manual as well as a beautiful garden book, presenting procedures Ken Druse has personally tested and adapted, as well as photographed step by step. “This is a book for all seasons, and will appeal to anyone intrigued by how plants grow.” —Virginia McClain Miller, Fine Gardening