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Medical BioMethods Handbook
John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.
La favorita del re
Galles, 1093. La vita della giovane Nesta, figlia del principe gallese Rhys di Deheubarth, viene sconvolta il giorno in cui il padre muore combattendo contro i normanni. Presa in ostaggio e condotta in Inghilterra alla corte di Guglielmo II, il suo onore a Palazzo è messo a dura prova quando è costretta a diventare concubina di Enrico, il fratello minore del re e futuro sovrano, e poi viene data in sposa a Geraldo FitzWalter, un devoto e ambizioso cavaliere, come premio per il meritevole servizio svolto a Pembroke, in Galles. Nonostante l’odio e la rabbia verso i normanni, Nesta vede in questo matrimonio la possibilità di tornare nelle sue terre e risollevare le sorti del suo popolo, onorando così la memoria del padre. Ma le prove per Nesta purtroppo non sono ancora terminate. Di lei si invaghisce Owain, il figlio carismatico, ma spietato e senza scrupoli di un potente principe gallese. Quando le offre la possibilità di unirsi a lui nel suo piano di riconquista dell’intero Galles, Nesta è costretta a scegliere se perseguire il suo progetto a qualunque costo, oppure ancora una volta sacrificare se stessa in nome del suo ruolo di sposa e di madre…
Unplugged (A Portrait of a Rock Star)
Tagg Holland, lead singer of the band Holland, is no stranger to the indulgent life of a rock idol. But when a one-night stand leads to a fatal accident, Tagg Holland leaves his celebrity behind and seeks life underground, running from everyone but the one person he can't outrun...Until Mara shows up at his door, training bag in hand. Suddenly life begins looking up for Tagg. With renewed hope, Tagg revisits his rock and roll life and attempts to rise above his old debaucheries...until another near-fatal accident threatens Tagg's resolve and sends him plummeting once again.With his emotional stability tinkering on the edge of another breakdown, will Tagg Holland be capable of finding his way back to the top...or will he be just another rock legacy who lived way too fast, dying way too young?
Dead Wrong
From New York Times bestselling author Mariah Stewart comes a novel of sexy romantic suspense for fans of Nora Roberts, Catherine Coulter, and Karen Robards. “Mariah Stewart is fast becoming a brand-name author.”—Romantic Times Three devious prisoners vow murderous revenge. Now the first is free. It was inescapably chilling, as if the murderer was methodically working his way down a page torn from the phone book. The three victims brutally killed in their own homes had one thing in common: they were all listed as M. Douglas. The fact that Mara Douglas is next on the list has her jumping at shadows, until FBI agent Aidan Shields shows up to make sure she doesn’t become the fourth victim. Aidan has been out of commission since an undercover operation went bad more than a year ago. Back on the job, his razor-sharp instincts are returning. But it will take all of Aidan’s wits to stay one step ahead of the elusive killer who has engaged him in a deadly game—a game in which Mara’s life is the prize. A game only one can win . . . BONUS: This edition includes an excerpt from Mariah Stewart's Dead Certain.
La cultura musicale degli italiani
Una riflessione del tutto inedita sull’entità e la qualità della consapevolezza musicale degli italiani lungo tutto il Novecento, che va oltre le limitazioni di “genere” e abbraccia una definizione il più ampia possibile di “cultura”: si prende in considerazione infatti non solo il “sapere” musicale strettamente inteso, dunque, ma - allargandosi su una declinazione di campo culturale più aggiornata dal punto di vista teoretico e storiografico – ogni tipo di esperienza che può configurarsi come pratica culturale. Le grandi direttrici di ricerca individuate sono tre: 1) la formazione musicale all’interno dei percorsi educativi istituzionali e i percorsi formativi non ist...
