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Industrial Applications
This volume gives a survey of the state of the art in the traditional fields of industrial mycology as well as of selected novel applications of fungi. The first section deals with the use of fungi in the production and processing of bread, cheese, beer and wine, traditional Asian fermentation products and edible mushrooms. The second section is devoted to the production of fungal metabolites and enzymes representing value-added products. In addition to antibiotics, alkaloids organic acids, vitamins and industrial enzymes, which have successfully been in use for decades, it is also dedicated to fungal metabolites, such as insecticidal and nematicidal compounds, immunosurppressants and flavor...
Grand Challenges in Fungal Biotechnology
This volume provides a comprehensive overview of the major applications and potential of fungal biotechnology. The respective chapters report on the latest advances and opportunities in each topic area, proposing new and sustainable solutions to some of the major challenges faced by modern society. Aimed at researchers and biotechnologists in academia and industry, it represents essential reading for anyone interested in fungal biotechnology, as well as those working within the broader area of microbial biotechnology. Written in an accessible language, the book also offers a valuable reference resource for decision-makers in government and at non-governmental organizations who are involved i...
A Novel Heme-Thiolate Peroxygenase AaeAPO and Its Implications for C-H Activation Chemistry
In this thesis, Xiaoshi Wang investigates the function and mechanism of a newly discovered heme-thiolate peroxygenase, AaeAPO. This enzyme class comes from Agrocybe aegerita and is used in the conversion of inert hydrocarbons to alcohols. Xiaoshi's work focuses on an extracellular P450 enzyme which is not limited in its stability and lack of solubility and therefore is relevant for widespread industrial use. The author demonstrates that the peroxygenase catalyzes a wide range of reactions. In some cases the author even describes very difficult transformations in molecules that are highly inert. Her detailed investigations provide a mechanistic framework for how the peroxygenase catalyzes such a large number of reactions. A major highlight of this thesis is the identification of key short-lived intermediates in the catalytic cycle of the peroxygenase, using rapid kinetic and spectroscopic methods, as well as the elucidation of the thermodynamic properties of these high-energy intermediates. This work adds new insight into an important class of enzymes.
Genetically Engineered Organisms in Bioremediation
Genetically Engineered Organisms in Bioremediation provides comprehensive coverage of biotechnological applications of genetically engineered microorganisms for the bioremediation of polluted environments. Chapters are contributed by international scientists with in-depth knowledge, expertise, vision and commitment in their scientific profession. They detail several genetically engineered microorganisms and their enzymes that could be applied to biologically break down persistent organic pollutants and recombinant DNA technologies which entail development of "suicidal-GEMs" for effective and safe remediation of heavily polluted sites. FEATURES: • Highlights genes that encode catabolic enzy...
Molds, Mushrooms, and Medicines
"From beneficial fungi that keep bacteria under control and consume dead cells to toxic molds that cause disease, our bodies are constantly navigating a fungus-filled world. And our interactions with fungi are not limited to what happens in our bodies as we interact with these microbes via our diet and as a source of medicines. In short, there exists an incredible range of interactions between humans and fungi. In this book, Nicolas Money describes this relationship between fungi and humans in the context of our health. Readers learn how the field of mycology has been advancing, from studies that reveal the diversity of yeasts that grow on the skin to research on the use of psychedelic mushr...
Environmental Biotechnology
A deeper insight into the complex processes involved in this field, covering the biological, chemical and engineering fundamentals needed to further develop effective methodologies. The book devotes detailed chapters to each of the four main areas of environmental biotechnology -- wastewater treatment, soil treatment, solid waste treatment, and waste gas treatment -- dealing with both the microbiological and process engineering aspects. The result is the combined knowledge contained in the extremely successful volumes 11a through 11c of the "Biotechnology" series in a handy and compact form.
Biohydrometallurgical Processes
Extensive industrialization has led to an increased release of toxic metals into the soil and air. Industrial waste can include mine overburden, bauxite residue, and E waste, and these can serve as a source of valuable recoverable metals. There are relatively simple methods to recycle these wastes, but they require additional chemicals, are expensive, and generate secondary waste that causes environmental pollution. Biohydrometallurgical processing is a cost-effective and ecofriendly alternative where biological processes help conserve dwindling ore resources and extract metals in a nonpolluting way. Microbes can be used in metal extraction from primary ores, waste minerals, and industrial a...
Practical Methods for Biocatalysis and Biotransformations 2
Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities. Practical Methods for Biocatalysis and Biotransformations 2 is a "how-to" guide that focuses on the practical applications of enzymes and strains of microorganisms that are readily obtained or derived from culture collections. The sources of starting materials and reagents, hints, tips and safety advice (where appropriate) are given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative m...
Monooxygenase, Peroxidase and Peroxygenase Properties and Mechanisms of Cytochrome P450
This book describes in 13 chapters mechanisms of P450 used to monooxygenate substrates via the NAD(P)H/O2 pathway using its peroxidase and peroxygenase functions. P450 also utilizes peroxides, peracids, periodate and iodosobenzene to oxygenate substrates via the shunt pathway. Also described are mechanisms used in the oxidation of pharmaceuticals by CYP3A4; acyl- carbon cleavage by CYP17A1, CYP19A1 and CYP51A1; metabolism of tetrabromodiphenyl ethers and bile acids by CYP2B6 and CYP3A4; metabolism of ω-6 and ω-3 polyunsaturated fatty acids; H2O2-mediated peroxygenation of substrates using substrate misrecognition; P450 oxidative reactions using electrochemical methods; electron transfer to P450 by redox proteins; hydroxylation of 1,8-cineole by P450cin; and peroxygenation by unspecific peroxygenases using H2O2. The topics covered are relevant to P450 researchers, professors and students from a variety of disciplines ranging from pharmacology, toxicology and microbiology to chemistry.
