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Electroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and cell types, and highly efficient. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. However, exposure of cells to brief, hi- intensity electric fields has found broad application in other aspects of biological research, and is now routinely used to introduce other types of biological and analytic molecules into cells, to induce cell...
This text provides comprehensive coverage of the latest research on enterococci.
In the pre-antibiotic era, the infections we currently see as relatively minor were sometimes deadly. This book discusses the rise (and sometimes fall) of “wonder drugs” designed to combat infections for which, at the time, there was no cure. However, history has shown us that, when antibiotics are overused, it can ultimately lead to either widespread discontinuation of the antibiotic or a drastic decrease in efficacy, leaving healthcare providers with few or no options for treatment. This book explores the discoveries of the major antibiotic drug classes, and highlights major bacterial strains which have become resistant and now represent difficult-to-treat infections. Barriers to new antibiotic development, as well as novel strategies for treating bacterial infections, are also explored.
Shamanism is commonly understood through reference to spirits and souls. However, these terms were introduced by Christian missionaries as part of the colonial effort of conversion. So, rather than trying to comprehend shamanism through medieval European concepts, this book examines it through ideas that started developing in the West after encountering Amerindian shamans. Microbes and Other Shamanic Beings develops three major arguments: First, since their earliest accounts Amerindian shamanic notions have had more in common with current microbial ecology than with Christian religious beliefs. Second, the human senses allow the unaided perception of the microbial world; for example, entopti...
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