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Organelle Contact Sites
This book provides the first comprehensive coverage of the quickly evolving research field of membrane contact sites (MCS). A total of 16 chapters explain their organization and role and unveil the significance of MCS for various diseases. MCS, the intracellular structures where organellar membranes come in close contact with one another, mediate the exchange of proteins, lipids, and ions. Via these functions, MCS are critical for the survival and the growth of the cell. Owing to that central role in the functioning of cells, MCS dysfunctions lead to important defects of human physiology, influence viral and bacterial infection, and cause disease such as inflammation, type II diabetes, neuro...
Enzymes Dependent on Pyridoxal Phosphate and Other Carbonyl Compounds as Cofactors
This volume is a summary of the proceedings of the 8th International Symposium on Vitamin B6 and Carbonyl Catalysis, held in Osaka, Japan, 15-19 October 1990. The development of molecular biology and x-ray crystallography techniques have contributed greatly to the knowledge and understanding of PLP-dependent enzymes. The structural, mechanistic and functional aspects of the PLP-dependent enzymes have been studied for over 40 years by many active researchers. Recent evidence indicates that the coenzyme is substituted by the pyruvoyl group which is a reactive carbonyl in some amino acid decarboxylases, and the presence of pyrroloquinoline quinone (PQQ), another carbonyl coenzyme, in other enzymes. The contents of this volume attest to the exciting progress in elucidating structure, mechanism of action, and the function of enzymes dependent on coenzymes containing the carbonyl group.
Golgi
This volume provides readers with a collection of new and classical methods, techniques, and applications used to address enduring questions about the structure and functions of the Golgi complex. The chapters in this volume cover diverse topics ranging from model systems; live and fixed cell imaging techniques; in vitro biochemical reconstitution systems; and specific methods developed to study Golgi formation, maintenance, and functions under physiological and pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Golgi: Methods and Protocols is a valuable tool for researchers in the field who wish to explore new areas of Golgi biology and for new investigators interested in exploring Golgi structure and function.
Molecular Aspects of Enzyme Catalysis
Der Umfang der Protein-Forschung nimmt rapide zu, die Techniken werden ständig verfeinert, was zu einer Explosion der Informationen auf diesem Gebiet geführt hat. Dieses Buch stellt die neuesten Ergebnisse der aktivsten Forscher auf dem Gebiet der Enzymkatalyse vor. 28 Autoren aus Japan, den USA und Israel geben unentbehrliche Informationen über Enzymstrukturen und Funktionsanalysen. Wertvolle Anregungen für die Neugestaltung wirkungsvoller Biokatalysatoren werden gegeben. Neue Trends und fortgeschrittene Techniken werden eingehend besprochen. Darüber hinaus werden die Strukturen und Funktionen wichtiger Enzyme im Detail dargestellt. Sie schließen ein . Aspartate Aminotransferase . Tryptophan Synthase . Alanine Racemase . Tryptophanase . Superoxid Dismutase . H+-ATPase
