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Low-Cost Methods for Molecular Characterization of Mutant Plants
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
COBIT 5 for Risk
Information is a key resource for all enterprises. From the time information is created to the moment it is destroyed, technology plays a significant role in containing, distributing and analysing information. Technology is increasingly advanced and has become pervasive in enterprises and the social, public and business environments.
A Systems Approach to Language Pedagogy
This volume represents the first attempt in the field of language pedagogy to apply a systems approach to issues in English language education. In the literature of language education, or more specifically, second or foreign language learning and teaching, each topic or issue has often been dealt with independently, and been treated as an isolated item. Taking grammar instruction as an example, grammatical items are often taught in a sequential, step-by-step manner; there has been no â€road map†in which the interrelations between the various items are demonstrated. This may be one factor that makes it more difficult for students to learn the language organically. The topics covered in this volume, including language acquisition, pedagogical grammar, and teacher collaboration, are viewed from a holistic perspective. In other words, language pedagogy is approached as a dynamic system of interrelations. In this way, â€emergent properties†are expected to manifest. This book is recommended for anyone involved in language pedagogy, including researchers, teachers, and teacher trainers, as well as learners.
Waldo and the Desert Island Adventure
Michael and his dog Waldo, walking along a rainy beach, pool their imaginations to share a possible adventure involving a band of pirates and several monsters.
Charting a Sustainable Future of ASEAN in Business and Social Sciences
This volume showcases selected conference papers addressing the sustainable future of ASEAN from the perspectives of business and social science disciplines. In addressing the 17 Sustainable Developments Goals (SDGs) envisioned by the United Nations in the domains of environment, health and well-being, posing potential means of reducing inequalities globally, the authors target specific issues and challenges confronting the fast-growing region of ASEAN and present suggestions for co-operation and commitment from governments, non-governmental organisations (NGOs) and society at large, in line with the ASEAN Vision 2020. Papers are selected from the 3rd International Conference on the Future o...
Routledge Handbook of Contemporary Indonesia
The Routledge Handbook of Contemporary Indonesia offers an overview of the modern making and contemporary dynamics of culture, society, and politics in this powerful Asian nation. It provides a comprehensive survey of key issues in Indonesian politics, economics, religion, and society.
Veil of Roses
Raised amidst the confines of Iranian society, young Tamila Soroush escapes the oppression of Iran for the freedom of America, enjoying her everyday acts of rebellion against her background and capturing her new life through the lens of her camera, all the while searching for a husband who can prevent her return home. Original.
