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Mitochondrial Genomics and Proteomics Protocols offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes such as apoptosis and cellular aging. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike. Part I introduces major experimental model systems and discusses their specific advantages and limitations for functional analysis of mitochondria. The concise overview of...
Mitochondria are the product of a long evolutionary history. It is now a well established fact that mitochondria did evolve from free living bacteria being the common ancestor of both, eukaryotic mitochondria and α-proteobacteria. Advances in genome sequencing, the establishment of in organello and in vitro assays to name only a few, contributed significantly to advances in plant mitochondrial research. Second generation sequencing and the ability to directly sequence and analyse the whole plant transcriptome certainly will help to develop the research on plant mitochondria to another level in the future. In this book the current knowledge about plant mitochondria is presented in a series o...
The gaseous molecule ethylene (C2H4), which is small in size and simple in structure, is a plant hormone most often associated with fruit ripening yet has a diversity of effects throughout the plant life cycle. While its agricultural effects were known even in ancient Egypt, the complexity of its mode of action and the broad spectrum of its effects and potential uses in plant physiology remain important scientific challenges today. In the last few decades, the biochemical pathway of ethylene production has been uncovered, ethylene perception and signaling have been molecularly dissected, ethylene-responsive transcription factors have been identified and numerous effects of ethylene have been described, ranging from water stress, development, senescence, reproduction plant-pathogen interactions, and of course, ripening. Thus ethylene is involved in plant development, in biotic and abiotic stress, and in reproduction. There is no stage in plant life that is not affected by ethylene, modulated by a complex and fascinating molecular machinery.
Phytoremediation is the process that uses plants to remove pollutants from soils. These pollutants are stored in the edible parts of plants and, if they are consumed above a certain level, they become a health risk for humans and animals. This book is a critical review of phytoremediation, its direct or indirect effects on food products, and the risks posed by this cost-effective technology in food safety. It shows how different plants are suited for phytoremediation, explains the role of toxicants in the environment, and analyses their effects and risks in the food chain at a global level. It also reviews the extraction methods of toxicants from plants after they are exposed to phytoremedia...
The field of proteomics has advanced considerably over the past two decades. The ability to delve deeper into an organism’s proteome, identify an array of post-translational modifications and profile differentially abundant proteins has greatly expanded the utilization of proteomics. Improvements to instrumentation in conjunction with the development of these reproducible workflows have driven the adoption and application of this technology by a wider research community. However, the full potential of proteomics is far from being fully exploited in plant biology and its translational application needs to be further developed. In 2011, a group of plant proteomic researchers established the ...
Mitochondrial biogenesis is an extremely complex process. A hint of this complexity is clearly indicated by the many steps and factors required to assemble the respiratory complexes involved in oxidative phosphorylation. These steps include the expression of genes present in both the nucleus and the organelle, intricate post-transcriptional RNA processing events, the coordinated synthesis, transport and assembly of the different subunits, the synthesis and assembly of co-factors and, finally, the formation of supercomplexes or respirasomes. It can be envisaged, and current knowledge supports this view, that plants have evolved specific mechanisms for the biogenesis of respiratory complexes. ...
This long-awaited second edition covers the major changes that have occurred in the field over the last decade Completely revised with the most up-to-date research and including brand new chapters, Annual Plant Reviews, Volume 50: Plant Mitochondria, 2nd Edition presents the multifaceted roles of mitochondria in plants. The book starts with a short history of plant mitochondrial research; discusses how coevolution shaped plant mitochondrial gene expression; explains control of number, shape, size, and motility of mitochondria; delves into stress responses and roles in stress alleviation in mitochondrial biochemistry; covers the damage repair pathway of the Calvin-Benson cycle; and more. Cont...