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Development and Employment of Untargeted and Targeted Tools for Virus Detection in the Frame of Water-based Epidemiology
Traditional methods of studying viruses are limited in their ability to detect novel pathogens, but recent advances in high-throughput sequencing (HTS) are changing that. HTS allows researchers to probe deeply into the virome of various hosts and environments, identifying known and unknown viral species. However, a targeted approach, like quantitative PCR, remains the golden standard for large-scale quantitative studies. The increasing popularity of water-based epidemiology (WBE) to monitor wastewater for pathogens has become particularly widespread with surveillance of SARS-CoV-2 during the COVID-19 pandemic. Optimizing this procedure demands an evaluation of the stability of the virus in w...
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