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HOW TO DETERMINE THE STRUCTURE AND FUNCTION OF GLYCOSYL HYDROLASES AND TREHALASES IN MYCOBACTERIUM TUBERCULOSIS AND ITS ROLE IN VIRULENCE.
Reports documented in biomedical literature indicates that Glycosyl hydrolases and trehalases in Mycobacteria especially in Mycobacterium tuberculosis may play a role in its virulence. Enzymatic assays are required to see if one can detect specific enzyme activity in M. tuberculosis through microbiological techniques, protein expression and purification, crystallization experiments, glycosyl hydrolase and trehalase assays and gene cloning which will help to determine the role of the enzyme in virulence. It will also enable us to identify the outer membrane protein required for the transport of lipopolysaccharides to the bacterial cell surface.
PRINCIPLES AND PROTOCOLS OF RESEARCH WRITING , ETHICS AND BIOETHICS FOR RESEARCHERS.
This book is an international coursebook on the guidelines, principles and protocols of research writing , ethics and bioethics for researchers. It also contains sections on how to write and publish a scientific paper and also how to write proposals for national and international funding.
BASIC PRINCIPLES AND FOUNDATIONS OF QUALITY CONTROL, QUALITY ASSURANCE AND EXTERNAL QUALITY ASSESSMENT IN A TB/HIV/AIDS REFERENCE LABORATORY.
Quality control is the measures that have to be included in each test run to verify that the test is working correctly. Quality control indicates whether a test run is valid. External quality assessment program is an external evaluation of laboratory performance based on participation in EQAP using proficiency panels.The key issues for TB/HIV/AIDS laboratory quality assurance is that there must be detailed standard operating procedures (SOP) with total compliance checklists for monitoring all activities, organizational schemes for processing, documentation and assessment, monitoring of staff (competency evaluation) with blind proficiencies, neat and complete documentation of all results, no deviation from procedures, maintaining of confidentiality, endorsement of safety measures, and identification of errors and addressing them with a corrective action plan.To have an effective quality plan, there must be focus on accuracy, detail, clarity and legibility. Check and recheck. Never assume.
A STEP BY STEP GUIDE TO PROFITABLE POULTRY FARMING FOR LOCAL CONSUMPTION AND EXPORT. An invaluable guide for farmers in poultry farming business.
This comprehensive poultry farming business training manual is an essential start-up procedure training for an individual that wants to go into poultry farming business . It is also an invaluable guide for commercial poultry farmers already into chicken egg production and other aspects of poultry farming as it revealed the well- guarded secrets of successful commercial poultry farmers and exporters of poultry products worldwide. This poultry farming business training manual is organized into 18 modules on poultry farming which consists of daily and weekly step-by-step procedures used in poultry farming operations to achieve the highest profit potential and also sources of loans to finance your poultry business.
BASIC PRINCIPLES AND FOUNDATIONS OF FIRE FIGHTING AND PREVENTION IN A TUBERCULOSIS AND HIV/AIDS REFERENCE LABORATORY.
This book examines the subject of fire prevention which has been of great significance and importance throughout man's history. This is because although laboratory scientists has learnt to use it every day for flaming, sterilization, decontamination, boiling, heating, incineration of wastes and other laboratory work, he has yet to learn how to control fire especially scientists in a tuberculosis (TB), human immune deficiency virus (HIV) and acquired immune deficiency syndrome (AIDS) reference laboratory. If fire is not properly controlled in the TB/HIV/AIDS reference laboratory, fire can destroy both the scientist and the TB/HIV/AIDS reference laboratory. It is therefore imperative that the knowledge of control and prevention of fire outbreak in the TB/HIV/AIDS reference laboratory must be pursued.
BASIC PRINCIPLES AND FOUNDATIONS OF BIOSAFETY AND LABORATORY SECURITY IN A TUBERCULOSIS AND HIV/AIDS REFERENCE LABORATORY.
This book examines the biological safety principles, characterization of four biosafety levels, areas of research needs, safety management programme profile, key tuberculosis and HIV/AIDS reference laboratory security protocols and the socio-economic implications of poor safety practices in a tuberculosis and HIV/AIDS reference laboratory. Laboratory safety programmes are plans for preventing sickness and injury to personnel and damage or destruction of physical assets. The fundamental objectives of a meaningful laboratory safety programme include improvement of safety skills and attitude of all personnel, and development of a surveillance programme for prompt identification of hazards. It also explains fully, the term "containment" which describes safe methods for managing infectious materials in the laboratory environment where they are being handled or maintained.
HOW TO GENOTYPE MYCOBACTERIUM TUBERCULOSIS ISOLATES
This training course book is an essential guide for laboratory scientists who wants to learn Mycobacterium tuberculosis genotyping . It is also an invaluable guide for mycobacteriologists who are already involved in tuberculosis genotyping as it reveals the well guarded secrets of efficient Mycobacterium tuberculosis genotyping and strain differentiation.This Mycobacterium tuberculosis genotyping training is a complete operational laboratory training covering the Methodology, Storage and precautions, Quality control, DNA extraction, Amplification, Amplification profile, Detection and Preparation of reagents for tuberculosis genotyping.
HOW TO DETERMINE THE EFFECT OF THE DELETION OF LCP GENES IN MYCOBACTERIUM TUBERCULOSIS ON ITS CELL WALL CHARACTERISTICS.
The cell wall is the most common target of anti-mycobacterial drugs and many compounds that are in clinical use or under development target enzymes that synthesize distinct layers of the cell wall.There is a crucial and urgent need to identify and characterize the enzymes that are responsible for making cell envelope in mycobacteria which will help in discovering new drug targets.Enzymatic assays for the identification and characterization of the enzymes that are responsible for making the cell envelope of M. tuberculosis and also the identification and characterization of the genomic islands of M. tuberculosis by gene cloning and sequencing.This will help to determine the features, composition , structure, properties and functions of the genes of M. tuberculosis. It will also help to know the impact and relevance of the LCP genes, their functions and role in cell envelope biogenesis.
HOW TO CONTROL THE TUBERCULOSIS AND HIV/AIDS EPIDEMIC
Many people, including those living with HIV, do not know that TB is curable. This is a basic message and one thing that is failing to get through. We should know how to get the message to policy- makers and funders that more drugs are needed for both diseases. It is not "either/or', there is no point providing ARVs only for patients to die of TB for lack of TB resources. Similarly, TB efforts alone are insufficient. HIV/AIDS is dramatically fuelling the TB epidemic in sub-Saharan Africa, where up to 70%% of TB patients are co-infected with HIV in some countries. For many years efforts to tackle TB and HIV have been largely separate, despite the overlapping epidemiology. Improved collaboration between TB and HIV/AIDS programmes will lead to more effective control of TB among HIV- infected people and to significant public health gains.
