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Guido, AKA The Sicilian Mustang in the porn circle always boasted of having a killer between his legs. He never realized how right he was until he noticed a swelling. Now he has two weeks to make right five years of lies and come clean once and for all with his friends, his family and his best friend s ex. And if that s not enough, his ruthless agent wants to get it all on tape and make a reality show out of it. Will he manage to clean his act before he becomes the Sicilian Gelding?
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Guido, AKA The Sicilian Mustang in the porn circle always boasted of having a killer between his legs. He never realized how right he was until he noticed a swelling. Now he has two weeks to make right five years of lies and come clean once and for all with his friends, his family and his best friends ex. And if thats not enough, his ruthless agent wants to get it all on tape and make a reality show out of it. Will he manage to clean his act before he becomes the Sicilian Gelding?
Provides background information and detailed protocols for developing a mouse colony and using the animals in transgenic and gene-targeting experiments. The protocols list the animals, equipment, and reagents required and step-by-step procedures. Topics include in vitro culture of preimplantation embryos, surgical procedures, the production of chimeras, and the analysis of genome alterations. The third edition adds protocols for cloning mice, modifying embryonic stem cells, intracytoplasmic sperm injection, and cryopreservation of embryos.
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