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Quantum Tunnelling in Enzyme-catalysed Reactions
In recent years, there has been an explosion in knowledge and research associated with the field of enzyme catalysis and H-tunneling. Rich in its breath and depth, this introduction to modern theories and methods of study is suitable for experienced researchers those new to the subject. Edited by two leading experts, and bringing together the foremost practitioners in the field, this up-to-date account of a rapidly developing field sits at the interface between biology, chemistry and physics. It covers computational, kinetic and structural analysis of tunnelling and the synergy in combining these methods (with a major focus on H-tunneling reactions in enzyme systems). The book starts with a ...
Oxidative Folding of Peptides and Proteins
With contributions from experts in the field, this book provides a comprehensive overview of the oxidative folding of cysteine-rich peptides.
Isoprenoid Synthesis in Plants and Microorganisms
Isoprenoids are important in primary and secondary metabolism. They have implications in a myriad of physiological processes notably in plants, microorganisms and parasites, and biological activities at the cellular, organism, and ecosystem levels. The importance of isoprenoids in various areas of the scientific world has spurred intense research worldwide. Also their role in "nutraceuticals" has stimulated scientific curiosity. Literature on isoprenoids is widely scattered in journals with quite differing readerships and geographic distribution. A comprehensive book on isoprenoids does not exist. Isoprenoid Synthesis in Plants and Microorganisms: New Concepts and Experimental Approaches fills this gap by presenting the latest and the most applicable information on isoprenoids. The most recent TERPNET conference serves as the backdrop and provides much of the inspiration for the topics covered in the book. Additional topics of interest are covered as well, making Isoprenoid Synthesis in Plants and Microorganisms: New Concepts and Experimental Approaches the most comprehensive review of isoprenoid synthesis to date.
Terpene Synthases
Terpene Synthases, Volume 700 in the Methods in Enzymology series, continues the legacy of this highly respected laboratory standard with its first dedicated collection on this important family of enzymes. Terpene synthases are a diverse set of enzymes that use exquisite mechanisms to form complex (poly)cyclic hydrocarbon skeletons. Chapters in this new volume include Structural analysis by X-ray crystallography and cryo-EM, Understanding mechanisms using stable isotopes, substrate analogs, or computational tools, Engineering fusion enzymes, Ancestral terpene cyclases, as well as the Sequence, structure, and function of non-canonical terpene synthases. - Presents the only collection of current methodology for the investigation of terpene synthases, with topics including from bioinformatics, enzymology, computational chemistry, and engineering - Includes chapters authored by international experts in the field - Provides the latest contributions in the leading serial Methods in Enzymology
Physical Biology of Proteins and Peptides
This book covers the latest developments in the physical biology of proteins and peptides. Key insights into microscopic and macroscopic approaches to describe biologically relevant macromolecules and their interactions are provided. This book also covers a wide range of tools, including theoretical methods as statistical mechanics, normal mode analysis, kinetic theory and stochastic processes, and all-atom and coarse-grained molecular dynamics simulations. New experimental techniques are also discussed, particularly related to amiloidogenic peptides and their mutations. This is an excellent book for molecular biologists, physicists, computational scientists, and chemists. It covers cutting-...
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Encyclopedia of Molecular Biology, Volume 1
Annotation The field of molecular biology has revolutionized the study of biology. The applications to medicine are enormous, ranging from diagnostic techniques for disease and genetic disorders, to drugs, to gene therapy. Focusing on the fundamentals of molecular biology and encompassing all aspects of the expression of genetic information, the Encyclopedia of Molecular Biology will become the first point of reference for both newcomers and established professionals in molecular biology needing to learn about any particular aspect of the field.
Measurement and Analysis of Kinetic Isotope Effects
Experimental Analysis of Enzyme Mechanism Using Isotope Effects, Volume 596, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this comprehensive update include Measurement of enzyme binding isotope effects, Chemical ligation and isotope labeling to locate dynamic effects, Measurement of heavy enzyme isotope effects, Extracting kinetic isotope effects from a global analysis of reaction progress curves, KIE of metabolic flux and enzymes, Solvent and Primary KIE on Flavin Enzymes, and The Rapid Determination of Primary Deuterium Isotope Effects on Enzyme-Catalyzed Proton Transfer at Carbon in 50/50 HOH/DOD. Readers who are interested in applying or understanding this research will find useful methods currently used for measuring isotope effects on solution and enzyme reactions. Written by pioneers of modern isotope effect research Is the only collection of modern kinetic isotope effect methods currently available
Enzymes in Synthetic Biology
Volume 608 of the series Methods in Enzymology covers key aspects of enzyme discovery, engineering tools and platforms, and examples of applications in the enzymology of synthetic biology. Detailed methods for laboratory use of enzymes in synthetic biology applications Informative case history examples illustrating how enzyme and metabolic engineering are used to generate new products Emphasises latest developments in laboratory automation for the engineering of biology Covers many aspects of the design, build, test, learn cycle used in synthetic biology
