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This volume provides detailed protocols for the identification and understanding of vasculogenic mimicry process in vitro and in vivo, in addition to protocols for microscopy and histology. Chapters guide readers through different materials, commercial and homemade scaffolds, Matrigel, cancer spheroids, 3D tissue constructs, vasculogenic processes, and mathematical model building. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Vasculogenic Mimicry: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Although a classmate says that she cannot play Peter Pan in the school play because she is black, Grace discovers her true identity, that she can do anything she sets her mind to do.
Holly Gibney surgiu tímida e reclusa em Mr. Mercedes e se tornou uma detetive particular talentosa. Neste romance inédito de Stephen King, ela retorna para enfrentar dois adversários perversos. Penny Dahl está desesperada para encontrar a filha, Bonnie, que sumiu sem deixar vestígios. Em busca de ajuda profissional, ela liga para a agência Achados e Perdidos, sob o comando de Holly Gibney. A detetive reluta em aceitar o caso, porque deveria estar de licença, mas algo na voz de Penny faz com que Holly não consiga ignorar o pedido. A poucos quarteirões de onde Bonnie foi vista pela última vez, moram Rodney e Emily Harris. Um casal de acadêmicos octogenários, dedicados um ao outro, ...
Optimization Techniques for Hybrid Power Systems: Renewable Energy, Electric Vehicles, and Smart Grid is a comprehensive guide that delves into the intricate world of renewable energy integration and its impact on electrical systems. With the current global energy crisis and the urgent need to address climate change, this book explores the latest advancements and research surrounding optimization techniques in the realm of renewable energy. This book has a focus on nature-inspired and meta-heuristic optimization methods, and it demonstrates how these techniques have revolutionized renewable energy problem-solving and their application in real-world scenarios. It examines the challenges and o...