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Development of the Visual System
Development of the Visual System presents a selection of current studies that clearly illustrate principles of visual system development. These range from retinal development in fish and frogs to the effects of abnormal visual experience on the primary visual cortex of the cat. The book is unique in addressing four specific and fundamental aspects of development: cell lineage and cell fate, specificity and targeting of axons, specification of visual cortex, and correlates of the critical period. Encompassing technical advances in cellular and molecular biology and in video imaging and microscopy, contributions in each of these areas provide new information at the cellular and molecular level...
Confocal Microscopy
In Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish. Centered in the many biological applications of the confocal microscope, the book makes possible the successful imaging of both fixed and living specimens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.
Developmental Neurobiology of Vision
This volume contains summaries of most of the invited presentations given by lecturers and participants at the NATO Advanced Study Institute, "Develop mental Neurobiology of Vision," held in Rethymnon, Crete, Greece 18-27 Sep tember 1978. The purpose of this meeting was to enable a relatively small international group of scientists and students to hold discussions and to present their views on current problems in the field. It was intended that the results of the exchanges would be conveyed to others in the native countries of the parti cipants. An attempt was made to obtain broad representation of disciplines within the area of the Institute and this is reflected in the diversity of the chapters in this book. Considerable interest has been generated in recent years concerning the development and plasticity of vision. Perhaps not unexpectedly, along with the high level of activity in this field, there have been so me controversial findings.
Methods in Cellular Imaging
Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), ...
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Methods in Avian Embryology
A current and authoritative guide, Methods in Avian Embryology presents a combination of classical embryological techniques and modern molecular biological approaches to studying the developing avian embryo. The only one of its kind, this book is specifically devoted to providing a detailed approach to studying avian embryos. It also describes how to use this system to study problems in cell, developmental, and neurobiology. The protocols emphasize microsurgery, histology, and cellular and molecular marking, which are not covered in the usual molecular biology methods manuals. The methods include: embryonic transplantations, cell culture and organ culture, in situ hybridization, classical histological techniques, and retrovirally mediated gene transfer. Key Features* Complete and easy-to-follow procedures* Helpful illustrations* Distinguished group of authors* Wide range of approaches
Avian Embryology
This revised edition will continue to serve as the most complete and up-to-date guide to the use of the avian embryo in studies of vertebrate development. It will include new approaches to analysis of the chick genome, gene knock-out studies using RNA interference, morpholinos, and other cutting edge techniques. As with the original edition, emphasis has been placed on providing practical guidance, highlighting potentials and pitfalls of all key cell biological and embryological techniques. *fully revised second edition *organized into basic and advanced Methods *new section on Functional Genomics
An Assessment of the National Science Foundation's Science and Technology Centers Program
The National Science Foundation requested that the Committee on Science, Engineering, and Public Policy of the NAS, the NAE, and the IOM form a panel to evaluate the accomplishments of the NSF Science and Technology Centers program (not individual centers) against its goals in research, education, and knowledge transfer. This report is the result of the work of the panel charged with that effort, and provides recommendations for moving forward.
