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This volume reviews various facets of Agrobacterium biology, from modern aspects of taxonomy and bacterial ecology to pathogenesis, bacterial cell biology, plant and fungal transformation, natural transgenics, and biotechnology. Agrobacterium-mediated transformation is the most extensively utilized platform for generating transgenic plants, but modern biotechnology applications derive from more than 40 years of intensive basic scientific research. Many of the biological principles established by this research have served as models for other bacteria, including human and animal pathogens. Written by leading experts and highlighting recent advances, this volume serves both as an introduction to Agrobacterium biology for students as well as a more comprehensive text for research scientists.
Agrobacterium is a plant pathogen which causes the “crown-gall” disease, a neoplastic growth that results from the transfer of a well-defined DNA segment (“transferred DNA”, or “T-DNA”) from the bacterial Ti (tumor-inducing) plasmid to the host cell, its integration into the host genome, and the expression of oncogenes contained on the T-DNA. The molecular machinery, needed for T-DNA generation and transport into the host cell and encoded by a series of chromosomal (chv) and Ti-plasmid virulence (vir) genes, has been the subject of numerous studies over the past several decades. Today, Agrobacterium is the tool of choice for plant genetic engineering with an ever expanding host range that includes many commercially important crops, flowers, and tree species. Furthermore, its recent application for the genetic transformation of non-plant species, from yeast to cultivated mushrooms and even to human cells, promises this bacterium a unique place in the future of biotechnological applications. The book is a comprehensive volume describing Agrobacterium's biology, interactions with host species, and uses for genetic engineering.
Volumes 1 and 2 of Transgenic Plants assemble important information on transgenic crops which has appeared scattered in many different publications. These two volumes are a significant milestone in plant/agricultural biology, promote the practical application of recombinant DNA technology, and assist in transforming the agricultural industry.
The growing body of information on bacteria pathogenic for humans, mammals and plants generated within the past ten years has shown the interesting conservation of newly identified genes that playa direct role in the pathogenic mechanism. In addition to these genes, there are also genes that confer host specificities and other traits important in pathogenesis on these pathogens. In this volume, we have organized the subject areas to best fit the concept on the way bacterial pathogens recognize, interact and invade the host, on the regulation of genes involved in virulence, on the genes involved in the elaboration of toxins and other pathogenic components such as iron sequestering proteins, and on the mechanisms of circumventing the host defense systems. These areas are divided into Sections. Section I covers the first step when the pathogen seeks its host, and Sections II through VI cover subsequent steps leading to pathogenesis while avoiding host defenses. We conclude this work with a chapter summarizing information on examples of virulence mechanisms that are highly conserved.
"Plant transformation technology has played a critical role in advancing biotechnology and fundamental research and evolved as a science. This book describes the breakthrough technologies in all aspects of plant transformation in the last 27 years, which "
This new and expanded Fourth Edition of Dr. Benarde's myth-defying book uses an evidence-based approach to refute today's pervasive, environmental doomsday hype. With up-to-date information consolidated from authoritative scientific journals and presented in highly readable form, Our Precarious Habitat . . . It's In Your Hands: covers hot-button issues like global warming, nuclear energy, electromagnetic fields, alternative health practices, and more; covers illnesses such as SARS, avian flu, West Nile virus, and prion diseases like mad cow; and includes facts, figures, tables, and charts derived from respected scientific sources and presented in easy-to-understand terms. New to this edition...
Before 1910 the American chestnut was one of the most common trees in the eastern United States. Although historical evidence suggests the natural distribution of the American chestnut extended across more than four hundred thousand square miles of territory—an area stretching from eastern Maine to southeast Louisiana—stands of the trees could also be found in parts of Wisconsin, Michigan, Washington State, and Oregon. An important natural resource, chestnut wood was preferred for woodworking, fencing, and building construction, as it was rot resistant and straight grained. The hearty and delicious nuts also fed wildlife, people, and livestock. Ironically, the tree that most piqued the e...