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Animal Cell Electroporation and Electrofusion Protocols
The ability to introduce macromolecules into animal cells, includ ing DNA, RNA, proteins, and other bioactive compounds has facili tated a broad range of biological studies, from biochemistry and biophysics to molecular biology, cell biology, and whole animal stud ies. Gene transfer technology in particular will continue to play an essential role in studies aimed at improving our understanding of the relationships between the gene structure and function, and it has impor tant practical applications in both biotechnology and biomedicine, as evidenced by the current intense interest in gene therapy. Although DNA and other macromolecules may be introduced into cells by a variety of methods, inc...
Yeast Systems Biology
Systems Biology aims at deciphering the genotype-phenotype relationships at the levels of genes, transcripts (RNAs), peptides, proteins, metabolites, and environmental factors participating in complex cellular networks in order to reveal the mechanisms and principles governing the behavior of complex biological systems. Yeast Systems Biology: Methods and Protocols presents an up-to-date view of the optimal characteristics of the yeast Saccharomyces cerevisiae as a model eukaryote, perspective on the latest experimental and computational techniques for systems biology studies, most of which were first designed for and validated in yeast, and selected examples of yeast systems biology studies ...
Current Catalog
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Trainees and Fellows Supported by the National Institute of Dental Research and Trained During Fiscal Year
Separate listings of trainees and fellows given grants and awards. Intended to identify young scientists who may be recruited for work in dental research by administrators and investigators. Contains 6 sections comprising listings of 2 groups by names, by major disciplines, and by dates of receipt of degrees. Entries include name, discipline, degrees and dates, year of birth, program director, and grant number.
Data-Centric Biology
In recent decades, there has been a major shift in the way researchers process and understand scientific data. Digital access to data has revolutionized ways of doing science in the biological and biomedical fields, leading to a data-intensive approach to research that uses innovative methods to produce, store, distribute, and interpret huge amounts of data. In Data-Centric Biology, Sabina Leonelli probes the implications of these advancements and confronts the questions they pose. Are we witnessing the rise of an entirely new scientific epistemology? If so, how does that alter the way we study and understand life—including ourselves? Leonelli is the first scholar to use a study of contemp...
Fungal Genomics
Through the integration of bioinformatic, genetic, transcriptomic, proteomic, metabolomic, phenomic and other massive datasets, genomics is revealing exciting new insights into fungal cell biology. The central theme of this volume is the strong impact that genomics is having upon our understanding of fungal biology, across a wide range of species, including model yeasts (such as Saccharomyces cerevisiae and Schizosaccharomyces pombe), filamentous fungi (such as Neurospora crassa and Aspergillus nidulans) and pathogenic fungi (such as Magnaporthe grisae, Candida albicans, Cryptococcus neoformans and Histoplasma capsulatum). World-renowned scientists address the following topics in these fungi: systems biology and evolution, circadian rhythms, apoptosis and stress responses, secretion, and environmental signalling networks. Particular emphasis is placed on fungal pathogenicity. Various genomic technologies are discussed, including genome-wide sequence comparisons, transcript profiling, proteomics, metabolomics and bioinformatics.
