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Euglena: Biochemistry, Cell and Molecular Biology
This much-needed book is the first definitive volume on Euglena in twenty-fire years, offering information on its atypical biochemistry, cell and molecular biology, and potential biotechnology applications. This volume gathers together contributions from well-known experts, who in many cases played major roles in elucidating the phenomenon discussed. Presented in three parts, the first section of this comprehensive book describes novel biochemical pathways which in some instances have an atypical subcellular localization. The second section details atypical cellular mechanisms of organelle protein import, organelle nuclear genome interdependence, gene regulation and expression that provides ...
Symbiosis
Symbiosis is the fourth volume in the series Cellular Origin and Life in Extreme Habitats (COLE). Fifty experts, from over a dozen countries, review their current studies on different approaches to these phenomena. The chapters present various aspects of symbiosis from gene transfer, morphological features, and biodiversity to individual organisms sharing mutual cellular habitats. The origin of the eukaryotic phase is discussed with emphasis on cyanelles, H syntrophy, N2 fixation, and S-based symbiosis (as well as the origin of mitochondrion, chloroplast, and nucleus). All members of the three domains of life are presented for sharing symbiotic associations. This volume brings the concept of living together as `One plus One (plus One) equals One.' The purpose of this book is to introduce the teacher, researcher, scholar, and student as well as the open-minded and science-oriented reader to the global importance of this association.
Immunoelectron Microscopy
Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal,...
High-Resolution Imaging of Cellular Proteins
This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluorescence microscopy and the third focuses on applications for transmission and scanning electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls thus ensuring successful results in the further study of this vital field.
Physiology
The Biology of Euglena, Volume III: Physiology covers areas of investigation on Euglena, focusing in particular on its physiology. The book demonstrates the wide utilization and research on the members of the genus, especially Euglena gracilis. The book discusses, in particular, the essential features of organelle distribution and structure in Euglena, and euglenoid surfaces. It presents circadian rhythms reported for Euglena, as well as the organism’s movement and locomotion. It also explains the various aspects of sensory responses of Euglena gracilis to photic, chemical, and mechanical stimuli. In addition, the book addresses the stimulation and inhibition of the metabolism and growth of Euglena gracilis. Lastly, the Euglena chloroplast membrane, including its structure, function, and photocontrol of its development are described.
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