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This volume covers a wide range of various fields of research, with the common thread being Next Generation Sequencing (NGS) related methods and applications, as well as analysis and interpretation of the data obtained. Chapters guide readers through the highly dynamic processes of translational and transcriptional profiling of a cell, method to detect copy number alterations (CNAs), targeted sequencing applications, method called “Hi-Plex” to characterize known polymorphic loci, single-cell of DNA or RNA, identify and characterize rare circulating CD4 T cells, and computational pipeline for RNAseq analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Next Generation Sequencing: Methods and Protocols aims to be useful and informative for further study into this vital field.
Otologic Surgery—the third edition of this invaluable surgical reference—has been thoroughly updated to reflect the latest advances in the field and covers all aspects of surgery of the ear and skull base. Derald E. Brackmann, MD, Clough Shelton, MD, and Moises A. Arriaga, MD bring you seven new chapters on the hot topics of Cartilage Tympanoplasty, Reversible Canal Wall Down Mastiodectomy, Superior Semicircular Canal Dehiscence Syndrome, Endoscopic Skull Base Surgery, Far Lateral Transcondylar Approach, Stereotactic Radiation Therapy, and Vascular Considerations in Neurotology. These extensive updates, along with the inclusion of new contributors and the elimination of redundant chapter...
With new medications, medical therapies, and increasing numbers of older and medically complex patients seeking dental care, all dentists, hygienists, and students must understand the intersection of common diseases, medical management, and dental management to coordinate and deliver safe care. This new second edition updates all of the protocols and guidelines for treatment and medications and adds more information to aid with patient medical assessments, and clearly organizes individual conditions under three headings: background, medical management, and dental management. Written by more than 25 expert academics and clinicians, this evidence-based guide takes a patient-focused approach to help you deliver safe, coordinated oral health care for patients with medical conditions. Other sections contain disease descriptions, pathogenesis, coordination of care between the dentist and physician, and key questions to ask the patient and physician.
Now in brilliant full color, Otologic Surgery, 4th Edition, by Drs. Derald Brackmann, Clough Shelton, and Moses A. Arriaga, offers comprehensive, step-by-step coverage of the full range of surgeries of the ear and skull base. Through crisp line drawings, high-quality photographs, and more than 50 procedural videos online, it provides detailed visual guidance that highlights the clinical expertise of dozens of the most respected specialists in the field. From cover to cover, you'll find the authoritative guidance you need to hone your surgical skills and ensure optimal outcomes for your patients. . Step-by-step coverage includes discussions of alternate approaches and controversial issues, as...
In the past decade, there has been an explosion of progress in understanding the roles of carbohydrates in biological systems. This explosive progress was made with the efforts in determining the roles of carbohydrates in immunology, neurobiology and many other disciplines, examining each unique system and employing new technology. This volume represents the second of three in the Methods in Enzymology series, including Glycobiology (vol. 415) and Functional Glycomics (vol. 417), dedicated to disseminating information on methods in determining the biological roles of carbohydrates. These books are designed to provide an introduction of new methods to a large variety of readers who would like to participate in and contribute to the advancement of glycobiology. The methods covered include structural analysis of carbohydrates, biological and chemical synthesis of carbohydrates, expression and determination of ligands for carbohydrate-binding proteins, gene expression profiling including micro array, and generation of gene knockout mice and their phenotype analyses.
Sataloffs Comprehensive Textbook of Otolaryngology (Six Volume Set) is a multi-volume textbook covering basic and clinical science across the entire field of otolaryngology. Volumes in the set include; otology, neurotology and skull-based surgery; rhinology, allergy and immunology; facial plastic and reconstructive surgery; laryngology; head and neck surgery; and paediatric otolaryngology. The full set is enhanced by over 5000 full colour images and illustrations, spanning nearly 6000 pages, complete with a comprehensive index on DVD. Edited by Robert T Sataloff from Drexel University College of Medicine, Philadelphia, each volume includes contributions from internationally recognised experts in otolaryngology, ensuring authoritative content throughout. Sataloffs Comprehensive Textbook of Otolaryngology (Six Volume Set) is an indispensable, in-depth guide to the field for all otolaryngology practitioners.
Soft Tissue Augmentation, 3rd Edition helps you make optimal use of these techniques and provide the optimum results your patients expect. Drs. Alistair and Jean Carruthers provide you with evidence-based, procedural how-to's and step-by-step advice on proper techniques, pitfalls, and tricks of the trade, equipping you to successfully incorporate the very latest procedures into your busy practice! Consult this title on your favorite e-reader, conduct rapid searches, and adjust font sizes for optimal readability. Compatible with Kindle®, nook®, and other popular devices. Offer your patients the best care and avoid pitfalls. Evidence-based findings and practical tips equip you with the knowl...
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