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This book is a comprehensive guide to apical periodontitis in root-filled teeth that covers not only all aspects of diagnosis and management but also epidemiology, etiology, consequences, clinical decision-making, and prognosis. There is a particular focus on the available surgical and nonsurgical methods of retreatment designed to restore healthy periapical conditions. Alternatives to retreatment are also explored, including a conservative approach involving monitoring of the diseased tooth without intervention and the option of extraction and replacement with an implant or a fixed prosthesis. The goal of root canal treatment is to save the natural tooth even when tissue breakdown has resul...
Whole genome amplification generates microgram quantities of genomic DNA starting from a sample of as little as a few femtograms and so is a vital technique when sample material is limited, as well as for high-throughput assays. Whole Genome Amplification: Methods Express is a comprehensive up-to-date laboratory manual for this key technique. It provides detailed step-by-step protocols as well as hints and tips for success and troubleshooting, taking readers through all aspects of whole genome amplification. This book is an essential practical guide for any researcher currently using PCR for genomic amplification or who wishes to do so in future.
The third edition of Textbook of Endodontology provides lucid scholarship and clear discussion of endodontic principles and treatment to dental students and dental practitioners searching for current information on endodontic theories and techniques. Completely revised and updated new edition Features six new chapters Provides pedagogical features to promote understanding Includes clinical case studies to put the information in the clinical context Illustrated in full color throughout with clinical images and detailed diagrams Offers interactive multiple-choice questions on a companion website
This volume offers readers a pragmatic approach to endodontic therapy for permanent molars, based on up-to-date evidence. All chapters were written by experts in the field, and focus on preparation for treatment, vital pulp therapy, access cavity preparation, root canal shaping, outcome assessment, retreatment, apical surgery, and specific aspects of restorations for root canal-treated molars. The role of micro-CT data in visualizing canal anatomy is compared to cone beam CT, and detailed information on current clinical tools, such as irrigation adjuncts and engine-driven preparation tools is provided. Important steps are illustrated in clinical photographs and radiographs, as well as by sch...
This book presents up-to-date recommendations for the prevention, diagnosis, and management of complications in endodontic surgical procedures, based on the best available scientific evidence. Common risks such as wound healing impairment, infection and bleeding are discussed and specific complications related to endodontic surgery, such as maxillary sinus involvement and damage to adjacent neurovascular structures, are reviewed. For each step of endodontic surgical procedures, surgical goals and possible outcomes are reviewed. Preoperative, intraoperative and postoperative risk factors for complications are identified and treatment options presented. Helpful decision-making algorithms, tables and flow charts complement the reader-friendly text.
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The premiere two-volume reference on revelations from studying complex microbial communities in many distinct habitats Metagenomics is an emerging field that has changed the way microbiologists study microorganisms. It involves the genomic analysis of microorganisms by extraction and cloning of DNA from a group of microorganisms, or the direct use of the purified DNA or RNA for sequencing, which allows scientists to bypass the usual protocol of isolating and culturing individual microbial species. This method is now used in laboratories across the globe to study microorganism diversity and for isolating novel medical and industrial compounds. Handbook of Molecular Microbial Ecology is the fi...