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Chemiluminescence immunoassay is now established as one of the best alternatives to conventional radioimmunoassay for the quantitation of low concentrations of analytes in complex samples. During the last two decades the technology has evolved into analytical procedures whose performance far exceeds that of immunoassays based on the use of radioactive labels. Without the constraints of radioactivity, the scope of this type of analytical procedure has widened beyond the confines of the specialist clinical chemistry laboratory to other disciplines such as microbiology, veterinary medicine, agriculture, food and environmental testing. This is the first work to present the topic as a subject in ...
This volume provides updates of this established field in both methods and applications, as well as advances in applications of the microarray method to biomarkers such as DNAs, RNAs, proteins, glycans and whole cells. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microarray Technology: Methods and Applications aims to ensure successful results in the further study of this vital field.
COVID-19 Diagnostic Technologies covers diagnostic tests and sensing technologies, their characteristics, and limitations for the detection and diagnosis of COVID-19. The book focuses on laboratory or portable based sensing technologies aims and the potential benefit of promising low-cost and portable strategies for accurate detection of the viral diseases. COVID-19 Diagnostic Technologies opens with introduction and reviews the virology of SARS-CoV-2. It goes on to discuss nucleic acid-based tests for viral RNA detection which covers PCR-based tests, Isothermal Nucleic Acid Amplification, DNA-microarray-based tests, and sequencing-based tests. Protein-based tests, which include neutralizing antibodies, serological tests, and antigenic tests, are also discussed. The book concludes with novel strategies for viral detection like emerging biosensors, nanotechnology, and the role of new digital technologies.
Ithasbeenstatedthatourknowledgedoublesevery20years,butthatmaybe an understatement when considering the Life Sciences. A series of discoveries and inventions have propelled our knowledge from the recognition that DNA isthegeneticmaterialtoabasicmolecularunderstandingofourselvesandthe living world around us in less than 50 years. Crucial to this rapid progress was thediscoveryofthedouble-helicalstructureofDNA,whichlaidthefoundation forallhybridizationbasedtechnologies. Thediscoveriesofrestrictionenzymes, ligases, polymerases, combined with key innovations in DNA synthesis and sequencing ushered in the era of biotechnologyas a new science with profound sociological and economic implications tha...
The editor and authors have produced an excellent book that will be extremely useful for all microbiologists. A recommended book for all microbiology laboratories.
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The book analyzes extensively al-Zamakhsharī’s tafsīr al Kashshāf within the framework of the Mu‘tazilites’ five principles: (usūl al-khamsa) of their theology. Andrew Lane in his book entitled “A Traditional Mu‘tazilite Qur’ān Commentary: The Kashshāf of Jār Allāh al-Zamakhsharī” states that al-Kashshāf is not a Mu‘tazilite tafsīr of the Qur’ān. This book has been written to prove that al-Zamakhsharī’s tafsīr is completely in accord with the Mu‘tazilites’ theology which is embodied in their five principles. The book is divided into two parts. Part I comprises of al Zamakhsharī’s biography, al-Kashshāf, and his methodology of tafsīr. Part II covers comprehensive analysis of the five principles: unity of God; justice; the promise and the threat of divine reward and punishment; the intermediate position between belief and unbelief; and enjoining what is right and forbidding what is wrong. The book concludes that al-Zamakhsharī’s al-Kashshāf is a Mu‘tazilite tafsīr completely adhering to the Mu‘tazilites’ theology.