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The academy
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Lorenzo Lotto
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Golden Gate Cloning
Zusammenfassung: This volume provides an overview of the latest protocols that cover the full range of in silico, in vitro, and in vivo approaches to help researchers plan, design, and perform Golden Gate cloning experiments. The chapters in this book cover topics such as Golden Gate cloning of standardized parts; modular DNA construct design for high-throughput Golden Gate Assembly; Golden Gate cloning of Synthetic CRISPR RNA spacer sequences; Golden Gate cloning in Actinobacteria; and YeastFab cloning of toxic genes and protein expression optimization in yeast. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Golden Gate Cloning: Methods and Protocols is a valuable resource for all researchers interested in learning more about this important and developing field. Chapter 3 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com
Studies and Texts
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Developmental Motor Disorders
Bringing together leading experts--and providing vital insights to guide clinical practice--this is the first volume to comprehensively address childhood motor disorders from a neuropsychological perspective. The book explores the neural and behavioral bases of movement disorders and summarizes current findings from applied research. Existing approaches to assessment and neuroimaging are critically examined, and new and innovative methods presented. Authors also synthesize the latest knowledge on motor difficulties associated with specific developmental and neurological problems: cerebral palsy; neuromuscular disease; autism; brain injury; disorders of coordination, speech, and written language; and more. Other important topics covered include psychosocial effects of motor skills impairments, frequently encountered comorbidities, and the status of available intervention approaches.
The Lost Venetian Church of Santa Maria Assunta dei Crociferi
Version: 1.1.2 DOI: 10.5281/zenodo.4284460 Original Repository (Zenodo): https://doi.org/10.5281/zenodo.4094821 This book investigates the history and decoration of one of the most important churches of Venice in the 16th century: Santa Maria Assunta dei Crociferi. Painters and sculptors of the stature of Titian, Tintoretto, Veronese, Palma il Giovane, Vittoria and Campagna all contributed major works of art, many of which survive in the present-day church of the Gesuiti. But as a result of the suppression of the order of the Crociferi (Crosiers, or Crutched Friars) in 1656, and of the subsequent demolition of their church, the art-historical significance of this ensemble had become largely ...
Subject Catalog
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