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Barley
This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Barley: Methods and Protocols serves as a valuable reference volume for cereal researchers and breeders by providing detailed protocols covering important traditional skills such as crossing and tissue culture through to the latest technologies for genotyping, expression analysis, and genome editing.
Transgenic Wheat, Barley and Oats
Understanding the physical and genetic structure of cereal genomes and how defined coding and non-coding regions interact with the environment to determine a phenotype are key to the future of plant breeding and agriculture. The production and characteri- tion of transgenic plants is a powerful reverse genetic strategy increasingly used in cereals research to ascribe function to defined DNA sequences. However, the techniques and resources required to conduct these investigations have, until recently, been difficult to achieve or totally lacking in wheat, barley and oat. This book brings together the l- est protocols for the transformation, regeneration and selection using both biolistic and ...
Plant Genome Editing with CRISPR Systems
This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.
Women in Microbiology
Many girls want to become scientists when they grow up, just like many boys do. But for these girls, the struggle to do what they love and to be treated with respect has been much harder because of the discrimination and bias in our society. In Women in Microbiology, we meet women who, despite these obstacles and against tough odds, have become scientific leaders and revered mentors. The women profiled in this collection range from historic figures like Alice Catherine Evans and Ruth Ella Moore to modern heroes like Michele Swanson and Katrina Forest. What binds all of these remarkable women are a passion for their work, a zest for life, a warm devotion to mentoring others—especially young...
Population, Agriculture, and Biodiversity
This timely collection of 15 original essays written by expert scientists the world over addresses the relationships between human population growth, the need to increase food supplies to feed the world population, and the chances for avoiding the extinction of a major proportion of the world's plant and animal species that collectively makes our survival on Earth possible. These relationships are highly intertwined, and changes in each of them steadily decrease humankind’s chances to achieve environmental stability on our fragile planet. The world population is projected to be nine to ten billion by 2050, signaling the need to increase world food production by more than 70 percent on the same amount of land currently under production—and this without further damaging our fragile environment. The essays in this collection, written by experts for laypersons, present the problems we face with clarity and assess our prospects for solving them, calling for action but holding out viable solutions.
Molecular Biomethods Handbook
An authoritative team of investigators illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced scientists fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included. The goal is to provide established scientists and novices who are new to these techniques with a deeper understanding of the widest variety of biotechniques and their applications.
Plant Genome Engineering
This volume provides readers with a collection of the latest protocols used to study plant genome editing and trait engineering. The chapters in this book are organized into five parts: genome engineering systems; machinery design and validation; delivery tools; generation and analysis of engineering materials; and crop genome engineering applications. The chapters cover topics such as methods of applying the popular CRISPR-Cas9 or CRISPR-Cas12 systems for editing genomes in different crop species, the use of small synthetic plastome for potato genome engineering, and the use of CRISPR-Cas9 for algal cell genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Genome Engineering: Methods and Protocols is a valuable tool for researchers interested in learning more about this developing and important field.
Biotechnology of Major Cereals
Biotechnology of Major Cereals will focus on the recent advances and future prospects in cereal biotechnology. The first part of the book will cover the world’s major cereals and focus on new developments and trends. The second part will be technology rather than species-led, detailing fundamental developments in technologies and significant target traits.
The White Woman on the Green Bicycle
Author of The Mermaid of Black Conch, Rathbone Folio Prize 2021 longlisted, Winner of the Costa Best Novel Award 2020 & Winner of the Costa Book of the Year 2020 When George and Sabine Harwood arrive in Trinidad from England George instantly takes to their new life, but Sabine feels isolated, heat-fatigued, and ill at ease with the racial segregation and the imminent dawning of a new era. Her only solace is her growing fixation with Eric Williams, the charismatic leader of Trinidad's new national party, to whom she pours out all her hopes and fears for the future in letters that she never brings herself to send. As the years progress, George and Sabine's marriage endures for better or worse. When George discovers Sabine's cache of letters, he realises just how many secrets she's kept from him - and he from her - over the decades. And he is seized by an urgent, desperate need to prove his love for her, with tragic consequences…
