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Arabidopsis has long been acknowledged as the 'Botanical Drosophila' with its small genome, low levels of repetitive DNA, small size and fast generation time it is an ideal molecular genetic tool for the analysis of development in higher plants. Arabidopsis: A Practical Approach provides an introduction to most of the key techniques required for the use of Arabidopsis as an experimental system. It gives a basic introduction to the optimal growth conditions and genetic resources available for Arabidopsis, how this material should be handled, maintained and used. Individual chapters describe strategies for the identification, mapping (using multi-marker lines and recombinant inbreds), and characterisation of different mutants by microscopy, molecular cytogenetics and gene expression analysis. Different cloning strategies, using transposons, T-DNA and map position are described in detail. Sequencing of the Arabidopsis genome will be completed in 2000 and bioinformatics are of key importance; the tools that are available and where they can be found on the Web are presented.
The Second Horseman of the Apocalypse has come to take peace from the earth and make men slay each other: A resurgent Russian Federation is about to begin a quest for world domination. Europe is in chaos. Long-standing alliances are shattering, and betrayal is the new paradigm. The United States is on the brink of civil war because of the actions of Zoe Wilson. Zoe, once a Marine Corps sniper, works as a mercenary for a secret organization bent upon fundamentally changing the country. Her mission: murder innocent black Muslims. Caught up in the turmoil are Bret Manley, the new lieutenant governor of Connecticut; EJ, his wife; and Mai Faca, Bret’s former wife and tong assassin. They’ve fo...
FOREWORD BY PROFESSOR PAUL SALKOVSKIS - You left the doctor's surgery before you could ask the things you really wanted to know. - You've googled your question about OCD and had 75 answers, all contradicting each other. - You asked your best friend - but they looked at you strangely. You have so many questions, but no idea where to start finding the answers. Here they are. In this book you'll find the definitive, expert responses to all your FAQs: On OCD. No question is too simple, too embarrassing, too rude or too offbeat to be included, and each one has been asked by thousands of people just like you. Will people judge me for my thoughts? Can hormones make OCD worse? Does anyone ruminate as much as me? All these questions, and hundreds more, are covered in this short but powerful, helpful, practical guide to managing your OCD. Read at your leisure, or dip in and out when you most need the support or to shine a light on the thoughts and feelings that are making you uncomfortable or unhappy, and to bring them out of the shadows so you can understand and accept them.
The heart wants what the heart wants… Web designer Zoe Wilson has two good reasons to avoid Cafferty ranch. First, she’s scared of horses. And second, she absolutely does not want to bump into her first love, Jake Cafferty. Too bad her best friend just happens to be Jake’s sister. When she asks Zoe to update the ranch’s outdated website, Zoe agrees—happy to lose herself in the digital world. But the more time she spends near Jake, the more all those feelings from years ago rear up in her heart, kicking like a wild bronco… Jake never told anyone, but Zoe broke his heart when they were kids. Now she’s back, and the gruff cowboy vows there’s no way he’ll let that happen again. But there’s something about Zoe that he just can’t resist, and he soon finds himself falling for her all over again… Hard. When Zoe gets accepted into a prestigious program, she doesn’t want to give up on their love. But Jake can’t bring himself to leave the ranch. Can these two broken-hearted souls finally come together? Or will they be torn apart for good…
Confessions of love is a collection of poems and photograph that expresses the various kinds of love. In love there is excitement, friendship, loneliness, joy, loss, fear. This collection is a reminder of how much the heart holds within the aesthetics of love and being loved. The love between a parent and their child, between lovers, between friends, but also the love of music, art, colour, texture, and more
Recovering at the ranch has never been so… vigorous. With a knee injury capable of ending his football career, all Charlie Wild should be focusing on is recovery. But that's the last thing on his mind when Dylan Rose walks into the small town hospital he’s healing at. She's stunningly beautiful…Too bad the gorgeous doctor seems immune to his charms. Dylan Rose isn’t looking for romance when she meets her newest patient. Sure, Charlie is charming, handsome and built like the star quarterback he is. But she knows better than to fall for a player. Yet over time, she begins to see that he’s more than just a cute jock. Charlie has heart. He’s also persistent as all get out, and no matter how hard she tries, she can’t ignore the sizzling attraction growing between them. But just when she’s starting to fall for the big lug, Charlie betrays her, putting himself and their relationship in danger. Can she forgive him? Or will Charlie have to add heartache to the list of injuries keeping him on the sidelines?
As the magazine of the Texas Exes, The Alcalde has united alumni and friends of The University of Texas at Austin for nearly 100 years. The Alcalde serves as an intellectual crossroads where UT's luminaries - artists, engineers, executives, musicians, attorneys, journalists, lawmakers, and professors among them - meet bimonthly to exchange ideas. Its pages also offer a place for Texas Exes to swap stories and share memories of Austin and their alma mater. The magazine's unique name is Spanish for "mayor" or "chief magistrate"; the nickname of the governor who signed UT into existence was "The Old Alcalde."