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In stem cell research there are several key methods that, once mastered, can be extremely powerful. These methods enable you to rigorously test hypotheses, compare results to "gold standards," and may even spur improvements to existing protocols. This book describes numerous methods to derive, manipulate, target, and prepare stem cells for clinical use. The methods described here help you derive and test human embryonic stem cells, analyze bone marrow stem cell function in vitro and in vivo, image a stem cell transplant, cryopreserve stem cells and differentiate stem cells using microscale tec.
The AACR Annual Meeting is the focal point of the cancer research community, where scientists, clinicians, other health care professionals, survivors, patients, and advocates gather to share the latest advances in cancer science and medicine. From population science and prevention; to cancer biology, translational, and clinical studies; to survivorship and advocacy; the AACR Annual Meeting highlights the work of the best minds in cancer research from institutions all over the world.
Offering a practical look into the field, this volume presents the science behind microscale device design and the engineering of its fabrication. Supported with dozens of full-color illustrations, this book offers you clear, step-by-step methods for the cell capture from whole blood, high-throughput study of transcriptional dynamics in living cells, temporal control of cell-cell interaction, nanoscale measurements of cellular forces, immobilizing living c. elegans, optical and electrical on-chip cell sorting and human-on-chip modeling of drug metabolism.
This practical book is part of the new Artech House Methods in Bioengineering series - volumes designed to offer detailed guidance on authoritative methods for addressing specific bioengineering challenges. Written and edited by recognized experts in the field, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome problems that may be encountered. This unique volume covers in detail the basic techniques and protocols of regenerative medicine, focusing on methods for cell transplantation. You explore a wide range of critical factors, such as grown tissues, cells, laboratory-made compounds, and engineered organs. The books helps you understand the many intricate steps involved in tissue engineering, cell isolation, progenitors and stem cell differentiation, and in vivo models for assessing new strategies.
Written and edited by recognized experts in the field, the new Artech House Methods in Bioengineering book series offers detailed guidance on authoritative methods for addressing specific bioengineering challenges. Offering a highly practical presentation of each topic, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome problems that may be encountered. This first-of-its-kind volume addresses the important challenge of organ preservation and reengineering. The book presents cutting-edge techniques for damaged livers and hearts via normothermic perfusion, hypothermic machine perfusion for the liver, kidney and pancreas, and imaging techniques to assess the viability of injured kidneys. Professionals and researchers also find methods to decellularize whole organs to create scaffolds for tissue engineering. Moreover, the book presents an approach to enhancing hepatocyte recovery from marginal livers.
"This cutting-edge volume provides a detailed look at the two main aspects of systems biology: the design of sophisticated experimental methods and the development of complex models to analyze the data. Focusing on methods that are being used to solve current problems in biomedical science and engineering, this comprehensive, richly illustrated resource shows you how to: design of state-of-the art methods for analyzing biological systems Implement experimental approaches for investigating cellular behavior in health and disease; use algorithms and modeling techniques for quantitatively describing biomedical problems; and integrate experimental and computational approaches for a more complete view of biological systems." --Book Jacket.
This practical book is part of the new Artech House Methods in Bioengineering series - volumes designed to offer detailed guidance on authoritative methods for addressing specific bioengineering challenges. This volume is focused on the materials involved with nanoscale bioengineering. Nanomaterials are quickly moving into the mainstream as a critical component of biological research. Filling a critical gap in the current literature, this new resource presents practical, step-by-step methods to help professionals synthesize, characterize, functionalize and apply the nanomaterial that is most suitable for handling a given nanoscale bioengineering problem. Written and presented by the best scientists and engineers in their respective fields, the authors offer a clear and detailed understanding of how to carry out a wide range of important methods in this area.
Written and edited by recognized experts in the field, the new Artech House Methods in Bioengineering book series offers detailed guidance on authoritative methods for addressing specific bioengineering challenges. Offering a highly practical presentation of each topic, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome problems that may be encountered. Providing alternatives to animal testing is one of the hottest topics in biomedical research, and this groundbreaking volume addresses this critical issue.
Tissue engineering is an emerging field that involves the combination of materials, cells, and other signals or growth factors to generate new tissue that can be used to repair or replace damaged tissues due to injury or disease. This groundbreaking volume presents the latest methods and protocols for systematically building tissues in 3D configuration outside the body, as well as providing techniques that modulate repair and regeneration processes that occur "in situ" (in their natural or original place).
Written and edited by recognized experts in the field, the new Artech House Methods in Bioengineering series offers detailed guidance on authoritative methods for addressing specific bioengineering challenges. Offering a highly practical presentation of each topic, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome problems that may be encountered. This unique volume presents leading-edge microfluidics methods used to handle, manipulate, and analyze cells, particles biological components (e.g., proteins and DNA) for microdiagnostics.