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Endocytic Components: Identification and Characterization
In step with the rapid growth of research into the biochemical and functional characterization of the endosome, Volume 19 surveys the recent advances in the methodological aspects of this field. Coverage includes the use of free flow electrophoresis to define endosome subpopulations, the endosomal compartments in rat hepatocytes, the role of endosomes in transmembrane signalling, and six additional articles.
Cholesterol and PI(4,5)P2 in Vital Biological Functions
Cholesterol is an essential component of the plasma membrane. Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), although a minor phospholipid, is the most abundant membrane phosphoinositide. Both lipids play key roles in a variety of cellular functions including as signalling molecules and major regulators of protein function. Studies on these important lipids have traditionally focused on the effect of each lipid individually. Accumulating evidence indicates, however, that these lipids may cross-regulate each other’s levels. Furthermore, it is becoming evident that cholesterol and PI(4,5)P2 can act together to modulate protein function and biological processes. This book provides an over...
Membrane Dynamics and Calcium Signaling
This book describes the newest discoveries on calcium signaling happening at the cellular and intracellular membranes, often exerted in so called microdomains. Calcium entry and release, its interaction with proteins and resulting events on proteins and organelles are comprehensively depicted by leading experts in the field. Knowledge about details of these highly dynamic processes rapidly increased in recent years, the book therefore provides a timely summary on the processes of calcium signaling and related membrane dynamics; it is aimed at students and researchers in biochemistry and cell biology.
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Recent Developments in Annexin Biology
Discovered over 40 years ago, the annexin proteins were found to be a structurally conserved subgroup of Ca2+-binding proteins. While the initial research on annexins focused on their signature feature of Ca2+-dependent binding to membranes, over the years, the biennial “Annexin” conference series has highlighted additional diversity in the functions attributed to the annexin family of proteins. The roles of these proteins now extend from basic science to biomedical research, and are being translated into clinical settings. Research on annexins involves a global network of researchers and the 10th biennial Annexin conference brought together over 80 researchers from ten European countries, USA, Brazil, Singapore, Japan, and Australia for 3 days in September 2019. In this conference, the discussions focused on two distinct themes — the role of annexins in cellular organization and health and disease. The articles published in this Special Issue cover these two main themes discussed at the conference, offering a glimpse into some of the notable findings in the field of annexin biology
Endosome Signaling Part B
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the second of two volumes on endosome signaling and includes chapters on such topics as measurement of entry into the endosomal compartment by multi-parametric image analysis, assessment of peptide internalization and endosomal signaling, and VEGF-A in endosomal signaling. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers endosome signaling Contains chapters on such topics as measurement of biological effects of endosomal proteolysis of internalized insulin and multi-vesicular endosome biogenesis.
