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Biophysics and the Challenges of Emerging Threats
Single-molecule techniques eliminate ensemble averaging, thus revealing transient or rare species in heterogeneous systems [1–3]. These approaches have been employed to probe myriad biological phenomena, including protein and RNA folding [4–6], enzyme kinetics [7, 8], and even protein biosynthesis [1, 9, 10]. In particular, immobilization-based fluorescence te- niques such as total internal reflection fluorescence microscopy (TIRF-M) have recently allowed for the observation of multiple events on the millis- onds to seconds timescale [11–13]. Single-molecule fluorescence methods are challenged by the instability of single fluorophores. The organic fluorophores commonly employed in sing...
NMR Spectroscopy and Computer Modeling of Carbohydrates
Enormous progress has been made in the development of nuclear magnetic resonance (MNR) instruments and techniques during the past 10 years. The application of NMR to carbohydrates and related compounds has opened up a several new possibilities. The present day dissemination of NMR instruments renders it possible for a larger group of glyco-scientists to record and interpret their own NMR spectra. A review of the potency of the techniques in this field is therforeappropriate.
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Enzymatic Technologies for Marine Polysaccharides
The bioactivity potential of marine polysaccharides has long been considered an underexploited aspect. These molecules found in seaweed, microalgae, bacteria, and animal fish (shellfish, mollusks, etc.) and the derived oligosaccharides need to be explored thoroughly with an interdisciplinary approach. They are an extraordinary source of chemical diversity, and the literature highlights many applicative fields, including the food industry, cosmetics, biomedicine, agriculture, environmental protection, wastewater management, etc. More recently, a new challenge has emerged: the exploitation of marine biomass as the source of sustainable energy to participate in the future replacement of fossil ...
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