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In the Crucible of Chronic Lyme Disease
Following completion of his medical training and a one-year stint as attending physician on Howard Champion's Surgical Critical Care Service and MedStar Unit at Washington Hospital Center in the District of Columbia, Kenneth Liegner, M.D. returned to Westchester County, home of his Alma Mater, New York Medical College, to start a private practice. Unwittingly, he had 'plunked himself down' in the heart of a burgeoning epidemic of Lyme disease. His patients confronted him with puzzling syndromes that defied 'tidy' formulations of the illness and thrust him in to a Maelstrom of medical controversy. Lyme disease, a new poorly understood disease, emerged hand in hand with the rise 'managed care'...
Genetic Methods for Diverse Prokaryotes
This new volume presents overviews of the very latest genetic approaches in a diverse range of prokaryotes. Divided into three sections, the topics include essential techniques for genetic analysis, case studies in which genetic methods in carefully chosen genera are described and approaches are used in the elucidation of specific phenomena. - Up-to-date chapters on essential techniques for genetic analysis in diverse bacteria - The use of plasmids, phages and transposons and their applications to new organisms - Genetic methods in medically and industrially important bacteria such as Mycobacteria, Neisseria, Bacteroides, Clostridia, and spirochaetes - Analysis of virulence in Helicobacter and Erwina - Genetic methods in Archae - Photosynthesis and respiration in Paracoccus and Rhodobacter - Bacillus subtilis sporulation
Molecular Biology of Spirochetes
Diseases produced by spirochetes, including Lyme borreliosis, syphilis and leptospirosis, are on the rise worldwide. This volume focuses on a series of state-of-the-art presentations of the research taking place in the laboratories of the contributors, and serves as an introduction to those individuals entering in the field of spirochete research.
Spirochete Biology: The Post Genomic Era
Spirochetes comprise a fascinating group of bacteria. Although diverse in terms of their habitat, ecology and infectivity for vertebrate and non-vertebrate hosts, they are often considered together because of their similar cellular morphologies. This volume brings together an international group of experts to provide essential insights into spirochete biology, with an emphasis on recent advances made possible by the availability of genome sequences. As such, it offers a valuable resource for microbiologists and other scientists with an interest in spirochete biology.
Lyme Borreliosis in Europe and North America
The facts about Lyme disease, from epidemiology to treatment to control Lyme borreliosis, popularly known as Lyme disease, remains the most commonly reported tick-transmitted infection in North America and Europe. A growing body of scientific and clinical evidence has facilitated early diagnosis and effective treatment. Nonetheless, there are a number of misconceptions about this infectious disease that have given rise to unproven, potentially dangerous alternative therapies. In Lyme Borreliosis in Europe and North America, a team of authors whose expertise spans basic research, epidemiology, and clinical practice has compiled evidence-based information on Lyme borreliosis. Presents all the ...
Electroporation Protocols for Microorganisms
Electroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and cell types, and highly efficient. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. However, exposure of cells to brief, hi- intensity electric fields has found broad application in other aspects of biological research, and is now routinely used to introduce other types of biological and analytic molecules into cells, to induce cell...
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Plant Cell Electroporation And Electrofusion Protocols
Gene transfer is an essential technology for improving our under standing of gene structure and function. Although there are many meth ods by which DNA may be introduced into cells—including heat and chemical treatments, and microinjection—electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi ated DNA transfer soon prompted researchers to investigate electroporation for tr...
