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This detailed volume covers diverse aspects of P2X7 receptor analysis, ranging from its molecular structure to related pharmacological and immunological tools, via its analysis in heterologous expression systems as well as assays using primary cells and whole animal models. After three introductory chapters that focus on its structure, ligands, and physiological functions, the book details the generation of antibody and nanobody tools for P2X7 receptors, provides protocols for the analysis of expressed P2X7 receptors with a focus on their electrophysiological analysis, as well as protocols for the investigation of P2X7 down-stream signaling in immune cells by flow cytometry. Mouse models and...
Have you ever been disappointed, oppressed, abused, or abandoned by those you loved, believed in, and trusted? Do you aspire to become a better parent, leader, and role model? Would you love to explore and experience the power, light, and life that come from being loved and loving and enjoy the complete fruits, joy, peace, assurance, and transformation that come with it? Then this book is a must-read! It reveals a genuine, profound, and very intimate and compassionate fatherchild relationship the foundation and source of immense self-esteem, self-confidence, joy, and peace. It is my utmost desire to let you know that just as I am privileged so you too are or can become.